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IFT81 Polyclonal antibody

IFT81 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, Canine and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11744-1-AP

Synonyms

CDV 1, CDV 1R, CDV1, CDV1R, IFT81



Tested Applications

Positive WB detected inmouse testis tissue, HEK-293 cells, human brain tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells, MDCK cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11744-1-AP targets IFT81 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, Canine samples.

Tested Reactivity human, mouse, Canine
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT81 fusion protein Ag2339
Full Name intraflagellar transport 81 homolog (Chlamydomonas)
Calculated molecular weight 676 aa, 80 kDa
Observed molecular weight 75-80 kDa
GenBank accession numberBC029349
Gene symbol IFT81
Gene ID (NCBI) 28981
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT particles are multi-subunit complexes of proteins that functions to move non-membrane-bound particles from the cell body to the tip of cilium or flagellum, then return them to the cell body. Transport towards the ciliary tip is regulated by the IFT complex B (IFT-B), consisting of at least 15 IFT proteins, in association with kinesin motors, whereas transport from the ciliary tip back to the base is executed by a dynein motor in association with the IFT complex A (IFT-A), currently known to be composed of six IFT proteins. IFT81 is a subunit of IFT complex B.It may play a role in development of the testis and spermatogenesis. There are some isoforms of IFT81 with 73-78 kDa and 43-50 kDa.

Protocols

Product Specific Protocols
WB protocol for IFT81 antibody 11744-1-APDownload protocol
IHC protocol for IFT81 antibody 11744-1-APDownload protocol
IF protocol for IFT81 antibody 11744-1-APDownload protocol
IP protocol for IFT81 antibody 11744-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

PLoS Genet

Spinocerebellar ataxia type 11-associated alleles of Ttbk2 dominantly interfere with ciliogenesis and cilium stability.

Authors - Emily Bowie
mouseWB

Biol Reprod

Intraflagellar transport protein 74 is essential for spermatogenesis and male fertility in mice‡.

Authors - Lin Shi
ratWB

Cytoskeleton (Hoboken)

Intraflagellar Transporter Protein 140 (IFT140), a component of IFT-A complex, is Essential for Male Fertility and Spermiogenesis in Mice.

Authors - Yong Zhang
humanIF

Methods Cell Biol

Immunofluorescent staining of septins in primary cilia.

Authors - M S Kim
mouseWB

Dev Biol

Intraflagellar transporter protein (IFT27), an IFT25 binding partner, is essential for male fertility and spermiogenesis in mice.

Authors - Yong Zhang
mouseWB

Biol Reprod

IFT25, an intraflagellar transporter protein dispensable for ciliogenesis in somatic cells, is essential for sperm flagella formation.

Authors - Hong Liu