|Positive WB detected in||mouse testis tissue, HEK-293 cells, human brain tissue, mouse brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||hTERT-RPE1 cells, MDCK cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11744-1-AP targets IFT81 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, Canine samples.
|Tested Reactivity||human, mouse, Canine|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IFT81 fusion protein Ag2339|
|Full Name||intraflagellar transport 81 homolog (Chlamydomonas)|
|Calculated molecular weight||676 aa, 80 kDa|
|Observed molecular weight||75-80 kDa|
|GenBank accession number||BC029349|
|Gene ID (NCBI)||28981|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT particles are multi-subunit complexes of proteins that functions to move non-membrane-bound particles from the cell body to the tip of cilium or flagellum, then return them to the cell body. Transport towards the ciliary tip is regulated by the IFT complex B (IFT-B), consisting of at least 15 IFT proteins, in association with kinesin motors, whereas transport from the ciliary tip back to the base is executed by a dynein motor in association with the IFT complex A (IFT-A), currently known to be composed of six IFT proteins. IFT81 is a subunit of IFT complex B.It may play a role in development of the testis and spermatogenesis. There are some isoforms of IFT81 with 73-78 kDa and 43-50 kDa.
|Product Specific Protocols|
|WB protocol for IFT81 antibody 11744-1-AP||Download protocol|
|IHC protocol for IFT81 antibody 11744-1-AP||Download protocol|
|IF protocol for IFT81 antibody 11744-1-AP||Download protocol|
|IP protocol for IFT81 antibody 11744-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Spinocerebellar ataxia type 11-associated alleles of Ttbk2 dominantly interfere with ciliogenesis and cilium stability.
Intraflagellar transport protein 74 is essential for spermatogenesis and male fertility in mice‡.
Intraflagellar Transporter Protein 140 (IFT140), a component of IFT-A complex, is Essential for Male Fertility and Spermiogenesis in Mice.
Methods Cell Biol
Immunofluorescent staining of septins in primary cilia.
Intraflagellar transporter protein (IFT27), an IFT25 binding partner, is essential for male fertility and spermiogenesis in mice.
IFT25, an intraflagellar transporter protein dispensable for ciliogenesis in somatic cells, is essential for sperm flagella formation.