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CoraLite®594-conjugated IFT81 Polyclonal antibody

IFT81 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, dog

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-11744

Synonyms

CDV 1, CDV 1R, CDV1, CDV1R, IFT81



Tested Applications

Positive IF detected inMDCK cells
For other applications, we recommend the unconjugated version of this antibody, 11744-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-11744 targets IFT81 in IF applications and shows reactivity with human, dog samples.

Tested Reactivity human, dog
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT81 fusion protein Ag2339
Full Name intraflagellar transport 81 homolog (Chlamydomonas)
Calculated molecular weight 676 aa, 80 kDa
GenBank accession numberBC029349
Gene symbol IFT81
Gene ID (NCBI) 28981
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT particles are multi-subunit complexes of proteins that functions to move non-membrane-bound particles from the cell body to the tip of cilium or flagellum, then return them to the cell body. Transport towards the ciliary tip is regulated by the IFT complex B (IFT-B), consisting of at least 15 IFT proteins, in association with kinesin motors, whereas transport from the ciliary tip back to the base is executed by a dynein motor in association with the IFT complex A (IFT-A), currently known to be composed of six IFT proteins. IFT81 is a subunit of IFT complex B.It may play a role in development of the testis and spermatogenesis. There are some isoforms of IFT81 with 73-78 kDa and 43-50 kDa.

Protocols

Product Specific Protocols
IF protocol for CL594 IFT81 antibody CL594-11744Download protocol
Standard Protocols
Click here to view our Standard Protocols