Anti-Mouse IGSF2/CD101 Rabbit Recombinant Antibody

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• Anti-Mouse IGSF2/CD101 Rabbit Recombinant Antibody (98596-2-RR)

• 

  FC experiment of mouse bone marrow cells using 98596-2-RR

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse IGSF2/CD101 Rabbit RecAb (98596-2-RR, Clone: 250569E6) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5) (APC-65140, Clone: RB6-8C5). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using 98596-2-RR

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse IGSF2/CD101 Rabbit RecAb (98596-2-RR, Clone: 250569E6) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98596-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98596-2-RR against Mouse IGSF2/CD101 were performed. The affinity constant is below 1 pM.

IGSF2/CD101 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98596-2-RR

Clone No.250569E6

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

CD101, EWI-101, Glu-Trp-Ile EWI motif-containing protein 101, IgSF2, Immunoglobulin superfamily member 2

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98596-2-RR targets IGSF2/CD101 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1832

  Product name: recombinant mouse IGSF2/CD101 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 21-970 aa of NM_001167906.1

  Sequence: QREVKIQEGPLYRAEGYPVSIRCTVSGHQGPSTQDFRWSIYLPSAPTKEVQIISTKDAGFSYAVYAQRVQSKEIYIERLQGDSVLLHISKLQMKDAGEYECHTPNTDGKYFGSYSAKTNLTVVPDTLSATMPSQTLSKKEGEPLELTCETTKATVQHTHLSLTWYLMQEGGGSQATEIVSLSKDFVLTPGSSYADRFVAGDVRLDKLGATSFRLSVGKLQPSDQGQVFCEATEWIQDPDETWTLITRKQTDQTALRIQPAARDFTVSITASSSPDEGKPLELVCLAVGRDGNPQLQGVWFLNGKEIAQTDAGGVLDLKRDYRDRASQGQLQVSKLSAQTFSLKIFSVGPEDVGTYSCEVAEVARTQMGSWQVLQRKQSPGYRVQLREPAARSVTVSAEQRTVWEGETLTLLCKAAGDVSALSVSWWLTPQDQSTPVFVAGMGQDGTVQLGVSSPGPAHRGNRRLEKVDWATFRLEIASAMVTDSGTYECRVSERLQNQAKGLQSTQKISVTVKSLKSSLRVNLMSRQPQVMLAHTFHLSCVVRANYSDLKLPFSVTWQFQPAGSGAFHRLIRIAHNGTVEWGDVLSQIHRKTKVSQSFFRSQLQIYDAAMEETGVYRCTVEVYDRDSICTSGPARVSATSNLLMITVTFPESKLSVNSSSQVQELSISSSTQIECAILSRSAGNLPLSIIWYFSSVSANASYLKILEMDQSSVVKYGDEFQTPRSKQKFYSEKVSQDLFLLNILSVEDSDQGHYHCAVEEWLLSTNDTWQKLERKTSGLTELKLRPTGSQVHVSKVNWTGNATEYGEAGFSCSLDGSGSTASLYSVTWYRGRGTATATAAAVANATATITAPAGSQMLVHLQYDGLLQYGREGSRRLQHCYRSSPTDFVLKLHRVEMEDAGIYWCRVTEWQQHGHPGKWINQASGESQRMVLRVLRSEPTVSSLICSSGP

• Full Name: immunoglobulin superfamily, member 2
• Calculated Molecular Weight: 114kDa
• GenBank Accession Number: NM_001167906.1
• Gene Symbol: Igsf2
• Gene ID (NCBI): 630146
• RRID: AB_3746204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: A8E0Y8
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

IGSF2, also known as CD101, is a low abundance protein. Expression of CD101 is found on monocytes, granulocytes and dendritic cells. In addition expression on T lymphocytes is important for cell activation. It plays a role as inhibitor of T-cells proliferation induced by CD3 and inhibits expression of IL2RA on activated T-cells and secretion of IL2.