IL-1 beta Recombinant monoclonal antibody

• 

  WB analysis of THP-1 using 82696-15-RR

  LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 82696-15-RR (IL-1 beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 82696-15-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82696-15-RR (IL1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of THP-1 using 82696-15-RR

  Immunofluorescent analysis of (4% PFA) fixed LPS and Brefeldin A treated THP-1 cells using IL-1 beta antibody (82696-15-RR, Clone: 240902D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 82696-15-RR

  Biolayer interferometry (BLl) kinetic assays of 82696-15-RR against Human IL-1 beta were performed. The affinity constant is 2.09 nM.

IL-1 beta Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 82696-15-RR

Clone No.240902D6

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

IL1- beta, IL1 beta, IL 1 beta, IL 1, Catabolin

---

Tested Applications

• Positive WB detected in: LPS and Brefeldin A treated THP-1 cells, A431 cells, HT-1080 cells, U-87 MG cells
• Positive IF/ICC detected in: LPS and Brefeldin A treated THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

82696-15-RR targets IL-1 beta in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Fusion Protein

• Full Name: interleukin 1, beta
• Calculated Molecular Weight: 269 aa, 31 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC008678
• Gene Symbol: IL1B
• Gene ID (NCBI): 3553
• ENSEMBL Gene ID: ENSG00000125538
• RRID: AB_3670529
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01584
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Med Rep; CTSB mediates oxidative stress and intestinal epithelial barrier disruption in intestinal ischemia‑reperfusion injury.; Authors - SHUANG HE
human	WB	Food Sci Nutr; Resveratrol Promotes Wound Healing by Enhancing Angiogenesis via Inhibition of Ferroptosis; Authors - Yujie Pan