IL-1 beta Recombinant antibody, PBS Only (Capture)
IL-1 beta Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
240902D6
Cat no : 82696-15-PBS
Synonyms
Validation Data Gallery
Product Information
82696-15-PBS targets IL-1 beta as part of a matched antibody pair:
MP00331-2: 82696-15-PBS capture and 82696-8-PBS detection (validated in Cytometric bead array, Sandwich ELISA)
MP00331-3: 82696-15-PBS capture and 82696-12-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Fusion Protein |
Full Name | interleukin 1, beta |
Calculated Molecular Weight | 269 aa, 31 kDa |
Observed Molecular Weight | 30-35 kDa |
GenBank Accession Number | BC008678 |
Gene Symbol | IL1B |
Gene ID (NCBI) | 3553 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
Background Information
Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.