FcZero-rAb® Biotin Anti-Human IL-4 Rabbit Recombinant Antibody

• 

  FC experiment of human PBMCs using Biotin-FcA98138

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Biotin Anti-Human IL-4 Rabbit RecAb (Biotin-FcA98138, Clone: 240794E11) and PE-conjugated streptavidin. Cells were co-stained with FcZero-rAb™ APC Anti-Human CD3 (OKT3) Rabbit IgG Recombinant Antibody (APC-FcA65569, Clone: OKT3). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

IL-4 FcZero-rAb^® Recombinant Antibody for FC (Intra), ELISA

Cat No. Biotin-FcA98138

Clone No.240794E11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Synonyms

IL4, B-cell stimulatory factor 1, BSF-1, IL 4, interleukin 4

---

Tested Applications

• Positive FC (Intra) detected in: PMA, Ionomycin and Brefeldin A treated human PBMCs

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA98138 targets IL-4 in FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0104

  Product name: Recombinant Human IL-4 protein (Myc Tag, His Tag)

  Source: mammalian cells-derived, pHZ-KIsec

  Tag: Myc & 6*His

  Domain: 25-153 aa of BC070123

  Sequence: HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS

• Full Name: interleukin 4
• Calculated Molecular Weight: 153 aa, 17 kDa
• GenBank Accession Number: BC070123
• Gene Symbol: IL-4
• Gene ID (NCBI): 3565
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05112
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

IL-4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.