INO80 Monoclonal antibody

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  WB analysis using 68064-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68064-1-Ig (INO80 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68064-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68064-1-Ig (INO80 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 68064-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68064-1-Ig (INO80 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 68064-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68064-1-Ig (INO80 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of PC-12 using 68064-1-Ig

  1X10^6 PC-12 cells were intracellularly stained with 0.8 ug Anti-Human INO80 (68064-1-Ig, Clone:1F11C5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

INO80 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 68064-1-Ig

Clone No.1F11C5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

hINO80, EC:3.6.4.-, DNA helicase-related protein INO80, DNA helicase-related INO80 complex homolog 1, Chromatin-remodeling ATPase INO80

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells, RAW 264.7 cells, PC-12 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68064-1-Ig targets INO80 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20265

  Product name: Recombinant human INO80 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1217-1556 aa of BC146785

  Sequence: KQVTVYRLICKGTIEERILQRAKEKSEIQRMVISGGNFKPDTLKPKEVVSLLLDDEELEKKLRLRQEEKRQQEETNRVKERKRKREKYAEKKKKEDELDGKRRKEGVNLVIPFVPSADNSNLSADGDDSFISVDSAMPSPFSEISISSELHTGSIPLDESSSDMLVIVDDPASSAPQSRATNSPASITGSVSDTVNGISIQEMPAAGRGHSARSRGRPKGSGSTAKGAGKGRSRKSTAGSAAAMAGAKAGAAAASAAAYAAYGYNVSKGISASSPLQTSLVRPAGLADFGPSSASSPLSSPLSKGNNVPGNPKNLHMTSSLAPDSLVRKQGKGTNPSGGR

• Full Name: INO80 homolog (S. cerevisiae)
• Calculated Molecular Weight: 1556 aa, 177 kDa
• Observed Molecular Weight: 177 kDa
• GenBank Accession Number: BC146785
• Gene Symbol: INO80
• Gene ID (NCBI): 54617
• RRID: AB_2918805
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9ULG1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA helicase INO80, also namd as INO80 or hINO80, is a 1,556 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and one DBINO domain. INO80 co-localizes with PCMA at replication forks during S-phase in the nucleus and belongs to the SNF2 helicase family. INO80 is a component of the chromatin remolding INO80 complex and is widely expressed in many tissues, such as brain,liver and pancreas. INO80 is involved in transcription regulation.