INO80 Monoclonal antibody

INO80 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F11C5

Cat no : 68064-1-Ig

Synonyms

hINO80, INO80, INO80 homolog (S. cerevisiae), INO80A, INOC1, KIAA1259



Tested Applications

Positive WB detected inK-562 cells, HeLa cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells, RAW 264.7 cells, PC-12 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

68064-1-Ig targets INO80 in WB, IHC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen INO80 fusion protein Ag20265
Full Name INO80 homolog (S. cerevisiae)
Calculated molecular weight 1556 aa, 177 kDa
Observed molecular weight177 kDa
GenBank accession numberBC146785
Gene symbol INO80
Gene ID (NCBI) 54617
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

DNA helicase INO80, also namd as INO80 or hINO80, is a 1,556 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and one DBINO domain. INO80 co-localizes with PCMA at replication forks during S-phase in the nucleus and belongs to the SNF2 helicase family. INO80 is a component of the chromatin remolding INO80 complex and is widely expressed in many tissues, such as brain,liver and pancreas. INO80 is involved in transcription regulation.

Protocols

Product Specific Protocols
WB protocol for INO80 antibody 68064-1-IgDownload protocol
IHC protocol for INO80 antibody 68064-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols