CoraLite® Plus 488-conjugated INO80 Monoclonal antibody

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  FC experiment of PC-12 using CL488-68064

  1X10^6 PC-12 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human INO80 (CL488-68064, Clone:1F11C5) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

INO80 Monoclonal Antibody for FC (Intra)

Cat No. CL488-68064

Clone No.1F11C5

Host / Isotype

Mouse / IgG1

Reactivity

rat

Applications

FC (Intra)

Synonyms

hINO80, EC:3.6.4.-, DNA helicase-related protein INO80, DNA helicase-related INO80 complex homolog 1, Chromatin-remodeling ATPase INO80

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Tested Applications

• Positive FC (Intra) detected in: PC-12 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68064 targets INO80 in FC (Intra) applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20265

  Product name: Recombinant human INO80 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1217-1556 aa of BC146785

  Sequence: KQVTVYRLICKGTIEERILQRAKEKSEIQRMVISGGNFKPDTLKPKEVVSLLLDDEELEKKLRLRQEEKRQQEETNRVKERKRKREKYAEKKKKEDELDGKRRKEGVNLVIPFVPSADNSNLSADGDDSFISVDSAMPSPFSEISISSELHTGSIPLDESSSDMLVIVDDPASSAPQSRATNSPASITGSVSDTVNGISIQEMPAAGRGHSARSRGRPKGSGSTAKGAGKGRSRKSTAGSAAAMAGAKAGAAAASAAAYAAYGYNVSKGISASSPLQTSLVRPAGLADFGPSSASSPLSSPLSKGNNVPGNPKNLHMTSSLAPDSLVRKQGKGTNPSGGR

• Full Name: INO80 homolog (S. cerevisiae)
• Calculated Molecular Weight: 1556 aa, 177 kDa
• Observed Molecular Weight: 177 kDa
• GenBank Accession Number: BC146785
• Gene Symbol: INO80
• Gene ID (NCBI): 54617
• RRID: AB_2934618
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9ULG1
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DNA helicase INO80, also namd as INO80 or hINO80, is a 1,556 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and one DBINO domain. INO80 co-localizes with PCMA at replication forks during S-phase in the nucleus and belongs to the SNF2 helicase family. INO80 is a component of the chromatin remolding INO80 complex and is widely expressed in many tissues, such as brain,liver and pancreas. INO80 is involved in transcription regulation.