INO80 Monoclonal antibody, PBS Only

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  WB analysis using 68064-1-Ig (same clone as 68064-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68064-1-Ig (INO80 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68064-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68064-1-Ig (same clone as 68064-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68064-1-Ig (INO80 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 68064-1-Ig (same clone as 68064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68064-1-Ig (INO80 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 68064-1-Ig (same clone as 68064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68064-1-Ig (INO80 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68064-1-PBS in a different storage buffer formulation.

• 

  FC experiment of PC-12 using 68064-1-Ig (same clone as 68064-1-PBS)

  1X10^6 PC-12 cells were intracellularly stained with 0.8 ug Anti-Human INO80 (68064-1-Ig, Clone:1F11C5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68064-1-PBS in a different storage buffer formulation.

INO80 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Cat No. 68064-1-PBS

Clone No.1F11C5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

hINO80, EC:3.6.4.-, DNA helicase-related protein INO80, DNA helicase-related INO80 complex homolog 1, Chromatin-remodeling ATPase INO80

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Product Information

68064-1-PBS targets INO80 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20265

  Product name: Recombinant human INO80 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1217-1556 aa of BC146785

  Sequence: KQVTVYRLICKGTIEERILQRAKEKSEIQRMVISGGNFKPDTLKPKEVVSLLLDDEELEKKLRLRQEEKRQQEETNRVKERKRKREKYAEKKKKEDELDGKRRKEGVNLVIPFVPSADNSNLSADGDDSFISVDSAMPSPFSEISISSELHTGSIPLDESSSDMLVIVDDPASSAPQSRATNSPASITGSVSDTVNGISIQEMPAAGRGHSARSRGRPKGSGSTAKGAGKGRSRKSTAGSAAAMAGAKAGAAAASAAAYAAYGYNVSKGISASSPLQTSLVRPAGLADFGPSSASSPLSSPLSKGNNVPGNPKNLHMTSSLAPDSLVRKQGKGTNPSGGR

• Full Name: INO80 homolog (S. cerevisiae)
• Calculated Molecular Weight: 1556 aa, 177 kDa
• Observed Molecular Weight: 177 kDa
• GenBank Accession Number: BC146785
• Gene Symbol: INO80
• Gene ID (NCBI): 54617
• RRID: AB_2918805
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9ULG1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DNA helicase INO80, also namd as INO80 or hINO80, is a 1,556 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and one DBINO domain. INO80 co-localizes with PCMA at replication forks during S-phase in the nucleus and belongs to the SNF2 helicase family. INO80 is a component of the chromatin remolding INO80 complex and is widely expressed in many tissues, such as brain,liver and pancreas. INO80 is involved in transcription regulation.