INS Polyclonal antibody

INS Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 15848-1-AP

Synonyms

ILPR, INS, IRDN



Tested Applications

Positive IHC detected inhuman pancreas tissue, mouse pancreas tissue, rat pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman pancreas tissue, mouse pancreas tissue, rat pancreas tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15848-1-AP targets INS in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INS fusion protein Ag8630
Full Name INS
Calculated Molecular Weight 110 aa, 12 kDa
GenBank Accession NumberBC005255
Gene Symbol INS
Gene ID (NCBI) 3630
RRIDAB_10597100
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INS is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. INS is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form INS, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in INS results in type 1 diabetes mellitus.

Protocols

Product Specific Protocols
IHC protocol for INS antibody 15848-1-APDownload protocol
IF protocol for INS antibody 15848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell

Long-Term Expansion of Pancreatic Islet Organoids from Resident Procr+ Progenitors.

Authors - Daisong Wang
mouseIF

Nat Protoc

Isolation of mouse pancreatic islet Procr+ progenitors and long-term expansion of islet organoids in vitro.

Authors - Jingqiang Wang
mouseIF

Autophagy

Secretory autophagy promotes RAB37-mediated insulin secretion under glucose stimulation both in vitro and in vivo

Authors - Shan-Ying Wu
mouseIHC

Cell Rep

Spatiotemporal role of SETD2-H3K36me3 in murine pancreatic organogenesis

Authors - Ping Lu
mouseIHC

Theranostics

Circular RNA circPPM1F modulates M1 macrophage activation and pancreatic islet inflammation in type 1 diabetes mellitus.

Authors - Caiyan Zhang
mouseIF

ACS Appl Mater Interfaces

Localized Controlled Release of Bilirubin from β-Cyclodextrin-Conjugated ε-Polylysine To Attenuate Oxidative Stress and Inflammation in Transplanted Islets.

Authors - Qing Yao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Boyan (Verified Customer) (05-14-2021)

very good for IF

  • Applications: Immunofluorescence
FH

Isha (Verified Customer) (01-06-2021)

Worked really well with mice pancreas. Selectively stained islets beta cells, no background staining

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Pancreas