|Positive IHC detected in||human pancreas tissue, mouse pancreas tissue, rat pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human pancreas tissue, rat pancreas tissue|
|Immunohistochemistry (IHC)||IHC : 1:500-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15848-1-AP targets INS in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||INS fusion protein Ag8630|
|Calculated molecular weight||110 aa, 12 kDa|
|Observed molecular weight||36 kDa|
|GenBank accession number||BC005255|
|Gene ID (NCBI)||3630|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
INS is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. INS is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form INS, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in INS results in type 1 diabetes mellitus.
Horm Metab Res
Tendons from non-diabetic humans and rats harbor a population of insulin-producing, pancreatic beta cell-like cells.
Gen Comp Endocrinol
The MEK Inhibitor Trametinib Suppresses Major Histocompatibility Antigen-mismatched Rejection Following Pancreatic Islet Transplantation.
Islet transplantation in the subcutaneous space achieves long-term euglycaemia in preclinical models of type 1 diabetes.
Differential protein analysis of serum exosomes post-intravenous immunoglobulin therapy in patients with Kawasaki disease.