CoraLite®594-conjugated INS Polyclonal antibody

INS Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Publications

1

Cat no : CL594-15848

Synonyms

ILPR, INS, insulin, IRDN

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Validation Data Gallery

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  IF Staining of mouse pancreas using CL594-15848

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite®594 INS antibody (CL594-15848) at dilution of 1:200, CoraLite®488 Glucagon antibody (CL488-15954, green).

• 

  IF Staining of mouse pancreas using CL594-15848

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite®594 INS antibody (CL594-15848) at dilution of 1:200, CoraLite®488 Glucagon antibody (CL488-15954, green).

• 

  IF Staining of human pancreas cancer using CL594-15848

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using CoraLite®594 INS antibody (CL594-15848) at dilution of 1:200.

• 

  IF Staining of human pancreas cancer using CL594-15848

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using CoraLite®594 INS antibody (CL594-15848) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: mouse pancreas tissue, human pancreas cancer tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL594-15848 targets INS in IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: INS fusion protein Ag8630
• Full Name: INS
• Calculated Molecular Weight: 110 aa, 12 kDa
• GenBank Accession Number: BC005255
• Gene Symbol: INS
• Gene ID (NCBI): 3630
• RRID: AB_2919824
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INS is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. INS is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form INS, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in INS results in type 1 diabetes mellitus.

Publications

Species	Application	Title
mouse	IF	Food Funct; Lycopene ameliorates islet function and down-regulates the TLR4/MyD88/NF-κB pathway in diabetic mice and Min6 cells; Authors - Yage Liu