INSIG1 Polyclonal antibody, PBS Only

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  WB analysis using 55282-1-AP (same clone as 55282-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55282-1-AP (INSIG1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55282-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 55282-1-AP (same clone as 55282-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55282-1-AP (INSIG1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55282-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 55282-1-AP (same clone as 55282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55282-1-AP (INSIG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55282-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 55282-1-AP (same clone as 55282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55282-1-AP (INSIG1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55282-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 55282-1-AP (same clone as 55282-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using INSIG1 antibody (55282-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 55282-1-PBS in a different storage buffer formulation.

INSIG1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 55282-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CL 6, INSIG 1, INSIG-1, insulin induced gene 1, Insulin induced gene 1 protein

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Product Information

55282-1-PBS targets INSIG1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: insulin induced gene 1
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: NM_005542
• Gene Symbol: INSIG1
• Gene ID (NCBI): 3638
• RRID: AB_2881298
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15503
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Insulin-induced genes (INSIGs), which consist of two isoforms (INSIG1 and INSIG2), are representative endoplasmic reticulum (ER)-resident oxysterol-binding proteins (OSBPs). INSIG1 is part of negative regulatory feedback, acting as a brake on SREBP transcriptional function (PMID: 23919961). INSIG1 also has a secondary function mediating sterolaccelerated proteolytic degradation of HMG CoA reductase, thus impacting the mevalonate pathway. Suppression of INSIG1 function promotes hepatic lipid remodelling and restrains NASH (non-alcoholic steatohepatitis) progression(PMID: 33722690).