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INSIG2 Polyclonal antibody
INSIG2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 24766-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | rat liver tissue, A549 cells, SGC-7901 cells |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 4 publications below |
WB | See 6 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
IP | See 2 publications below |
Product Information
The immunogen of 24766-1-AP is INSIG2 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | INSIG2 fusion protein Ag14072 |
Full Name | INSIG 2 |
Calculated molecular weight | 225 aa, 25 kDa |
Observed molecular weight | 30 kDa |
GenBank accession number | BC022475 |
Gene symbol | INSIG2 |
Gene ID (NCBI) | 51141 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
INSIG2 mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. INSIG2 may bring HMGCR into ubiquitin-mediated proteasomal degradation. Insig2 protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi. INSIG-2 may play an important role in therapy of hypercholesterolemia.
Protocols
Product Specific Protocols | |
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WB protocol for INSIG2 antibody 24766-1-AP | Download protocol |
IHC protocol for INSIG2 antibody 24766-1-AP | Download protocol |
IF protocol for INSIG2 antibody 24766-1-AP | Download protocol |
IP protocol for INSIG2 antibody 24766-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nature The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.
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Mol Metab CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing.
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Int J Biol Sci The GR-gp78 Pathway is involved in Hepatic Lipid Accumulation Induced by Overexpression of 11β-HSD1. | ||
Am J Cancer Res p38α/S1P/SREBP2 activation by the SAM-competitive EZH2 inhibitor GSK343 limits its anticancer activity but creates a druggable vulnerability in hepatocellular carcinoma. | ||
Proteomes A Multi-Level Systems Biology Analysis of Aldrin's Metabolic Effects on Prostate Cancer Cells
| ||
Mol Biol Rep Insulin-induced gene 2 expression is associated with cervical adenocarcinoma malignant behavior
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