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  • KD/KO Validated

INSIG2 Polyclonal antibody

INSIG2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24766-1-AP

Synonyms

INSIG 2, INSIG2



Tested Applications

Positive WB detected inrat liver tissue, A549 cells, SGC-7901 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24766-1-AP is INSIG2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INSIG2 fusion protein Ag14072
Full Name INSIG 2
Calculated molecular weight 225 aa, 25 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC022475
Gene symbol INSIG2
Gene ID (NCBI) 51141
RRIDAB_2811285
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INSIG2 mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. INSIG2 may bring HMGCR into ubiquitin-mediated proteasomal degradation. Insig2 protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi. INSIG-2 may play an important role in therapy of hypercholesterolemia.

Protocols

Product Specific Protocols
WB protocol for INSIG2 antibody 24766-1-APDownload protocol
IHC protocol for INSIG2 antibody 24766-1-APDownload protocol
IF protocol for INSIG2 antibody 24766-1-APDownload protocol
IP protocol for INSIG2 antibody 24766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Nature

The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.

Authors - Daqian Xu
  • KD Validated
humanWB,IF

Mol Metab

CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing.

Authors - Han Zeng
  • KD Validated
mouseWB,IP

Int J Biol Sci

The GR-gp78 Pathway is involved in Hepatic Lipid Accumulation Induced by Overexpression of 11β-HSD1.

Authors - Mengliang Hu
humanWB

Am J Cancer Res

p38α/S1P/SREBP2 activation by the SAM-competitive EZH2 inhibitor GSK343 limits its anticancer activity but creates a druggable vulnerability in hepatocellular carcinoma.

Authors - Pei-Ming Yang
humanWB

Proteomes

A Multi-Level Systems Biology Analysis of Aldrin's Metabolic Effects on Prostate Cancer Cells

Authors - Carmen Bedia
  • KD Validated
mouseWB

Nat Commun

In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion

Authors - Hasan Vatandaslar