INSIG2 Polyclonal antibody

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  WB analysis of rat liver using 24766-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 24766-1-AP (INSIG2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 24766-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 24766-1-AP (INSIG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 24766-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 24766-1-AP (INSIG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 24766-1-AP

  IP result of anti-INSIG2 (IP:24766-1-AP, 4ug; Detection:24766-1-AP 1:500) with mouse liver tissue lysate 3600ug.

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  IHC staining of human liver using 24766-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24766-1-AP (INSIG2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 24766-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24766-1-AP (INSIG2 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 24766-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using INSIG2 antibody (24766-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

INSIG2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 24766-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Insulin-induced gene 2 protein, INSIG-2, INSIG 2

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Tested Applications

• Positive WB detected in: rat liver tissue, A549 cells, SGC-7901 cells
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Product Information

24766-1-AP targets INSIG2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: INSIG2 fusion protein Ag14072
• Full Name: INSIG 2
• Calculated Molecular Weight: 225 aa, 25 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC022475
• Gene Symbol: INSIG2
• Gene ID (NCBI): 51141
• RRID: AB_2811285
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y5U4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INSIG2 mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. INSIG2 may bring HMGCR into ubiquitin-mediated proteasomal degradation. Insig2 protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi. INSIG-2 may play an important role in therapy of hypercholesterolemia.

Publications

Species	Application	Title
human	WB,IP	Nature; The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.; Authors - Daqian Xu ; KD Validated
human	WB,IF	Mol Metab; CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing.; Authors - Han Zeng ; KD Validated
mouse	WB,IP	Int J Biol Sci; The GR-gp78 Pathway is involved in Hepatic Lipid Accumulation Induced by Overexpression of 11β-HSD1.; Authors - Mengliang Hu
human	WB	Am J Cancer Res; p38α/S1P/SREBP2 activation by the SAM-competitive EZH2 inhibitor GSK343 limits its anticancer activity but creates a druggable vulnerability in hepatocellular carcinoma.; Authors - Pei-Ming Yang
human	WB	Proteomes; A Multi-Level Systems Biology Analysis of Aldrin's Metabolic Effects on Prostate Cancer Cells; Authors - Carmen Bedia ; KD Validated
mouse	WB	Nat Commun; In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion; Authors - Hasan Vatandaslar