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INSIG2 Polyclonal antibody

INSIG2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 24766-1-AP

Synonyms

INSIG 2, INSIG2



Tested Applications

Positive WB detected inrat liver tissue, A549 cells, SGC-7901 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24766-1-AP is INSIG2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INSIG2 fusion protein Ag14072
Full Name INSIG 2
Calculated molecular weight 225 aa, 25 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC022475
Gene symbol INSIG2
Gene ID (NCBI) 51141
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

INSIG2 mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. INSIG2 may bring HMGCR into ubiquitin-mediated proteasomal degradation. Insig2 protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi. INSIG-2 may play an important role in therapy of hypercholesterolemia.

Protocols

Product Specific Protocols
WB protocol for INSIG2 antibody 24766-1-APDownload protocol
IHC protocol for INSIG2 antibody 24766-1-APDownload protocol
IF protocol for INSIG2 antibody 24766-1-APDownload protocol
IP protocol for INSIG2 antibody 24766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Cancer Res

p38α/S1P/SREBP2 activation by the SAM-competitive EZH2 inhibitor GSK343 limits its anticancer activity but creates a druggable vulnerability in hepatocellular carcinoma.

Authors - Pei-Ming Yang
humanWB,IP

Nature

The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.

Authors - Daqian Xu
  • KD Validated