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Integrin Alpha 5 Polyclonal antibody
Integrin Alpha 5 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 27224-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, HeLa cells, human placenta tissue, U2OS cells |
Positive IP detected in | human placenta tissue |
Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of 27224-1-AP is Integrin Alpha 5 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Integrin Alpha 5 fusion protein Ag26098 |
Full Name | integrin, alpha 5 (fibronectin receptor, alpha polypeptide) |
Calculated molecular weight | 115 kDa |
Observed molecular weight | 135-150 kDa |
GenBank accession number | NM_002205 |
Gene symbol | ITGA5 |
Gene ID (NCBI) | 3678 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for Integrin Alpha 5 antibody 27224-1-AP | Download protocol |
IHC protocol for Integrin Alpha 5 antibody 27224-1-AP | Download protocol |
IP protocol for Integrin Alpha 5 antibody 27224-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Boyan (Verified Customer) (01-25-2019) | By WB, it labels a band at the expected molecular weight, but this band is very likely not the real protein, because it is increased during adipocyte differentiation, which should be decreased during the process according to literature.By IF, PFA fixation, it labels the protein in the nucleus. But according to literature, it is localised in the cell surface and the Gogli.
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