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Integrin Alpha 5 Polyclonal antibody

Integrin Alpha 5 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 27224-1-AP

Synonyms

CD49e, FNRA, Integrin alpha 5, Integrin alpha F, ITGA5, VLA 5, VLA5A



Tested Applications

Positive WB detected inA549 cells, HeLa cells, human placenta tissue, U2OS cells
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27224-1-AP is Integrin Alpha 5 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Integrin Alpha 5 fusion protein Ag26098
Full Name integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
Calculated molecular weight 115 kDa
Observed molecular weight 140-150 kDa
GenBank accession numberNM_002205
Gene symbol ITGA5
Gene ID (NCBI) 3678
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Integrin Alpha 5 antibody 27224-1-APDownload protocol
IHC protocol for Integrin Alpha 5 antibody 27224-1-APDownload protocol
IP protocol for Integrin Alpha 5 antibody 27224-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (01-25-2019)

By WB, it labels a band at the expected molecular weight, but this band is very likely not the real protein, because it is increased during adipocyte differentiation, which should be decreased during the process according to literature.By IF, PFA fixation, it labels the protein in the nucleus. But according to literature, it is localised in the cell surface and the Gogli.

  • Applications: Western Blot,Immunofluorescence,