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ITGBL1 Polyclonal antibody

ITGBL1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30010-1-AP

Synonyms

Integrin beta like protein 1, ITGBL1, OSCP, TIED

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ITGBL1 Polyclonal antibody (30010-1-AP)

    WB analysis using 30010-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30010-1-AP (ITGBL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, SKOV-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30010-1-AP targets ITGBL1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ITGBL1 fusion protein Ag31989
Full Name integrin, beta-like 1 (with EGF-like repeat domains)
Calculated Molecular Weight 494 aa, 54 kDa
Observed Molecular Weight54 kDa
GenBank Accession NumberBC036788
Gene Symbol ITGBL1
Gene ID (NCBI) 9358
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrin beta-like protein 1 (ITGBL1), also named as TIED or OSCP, is a secreted protein that contains ten tandem EGF-like repeats strikingly similar to those found in the cysteine-rich "stalk-like" structure of integrin beta subunits (PMID: 10051402). ITGBL1 participates in the regulation of fibrogenesis via interactions with transforming growth factor beta 1 (PMID: 28262670). ITGBL1 is also involved in the development and metastasis of many tumors (PMID: 32211321; 29772438; 26060017; 31190876).

Protocols

Product Specific Protocols
WB protocol for ITGBL1 antibody 30010-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols