Integrin Beta 1/CD29 Recombinant monoclonal antibody

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  WB analysis using 87495-1-RR

  RAW 264.7 cells lysates were subjected to SDS PAGE followed by western blot with 87495-1-RR (ITB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 87495-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 87495-1-RR (Integrin Beta 1/CD29 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 87495-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 87495-1-RR (Integrin Beta 1/CD29 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of RAW 264.7 using 87495-1-RR

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Integrin Beta 1/CD29 antibody (87495-1-RR, Clone: 252764F11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity of 87495-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87495-1-RR against Mouse Integrin Beta 1/CD29 were performed. The affinity constant is 1.32 nM.

Integrin Beta 1/CD29 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 87495-1-RR

Clone No.252764F11

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CD29, Fibronectin receptor subunit beta, Integrin beta-1, Itgb1, VLA-4 subunit beta

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Tested Applications

• Positive WB detected in: RAW 264.7 cells
• Positive IHC detected in: mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87495-1-RR targets Integrin Beta 1/CD29 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg10460

  Product name: Recombinant mouse ITB1 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-Twin-Strep-Tag

  Domain: 10011455 of

  Sequence: \

• Full Name: integrin beta 1 (fibronectin receptor beta)
• Observed Molecular Weight: 130-140 kDa
• Gene Symbol: Itgb1
• Gene ID (NCBI): 16412
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P09055
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrin beta-1 (ITGB1), also named as CD29, is a single-chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSCs from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, with weaker expression on granulocytes, and is not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.