KCC2/SLC12A5 Polyclonal antibody

KCC2/SLC12A5 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 28724-1-AP

Synonyms

hKCC2, K Cl cotransporter 2, KCC2, KCC2/SLC12A5, KIAA1176, Neuronal K Cl cotransporter, SLC12A5

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Validation Data Gallery

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  WB analysis using 28724-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 28724-1-AP (KCC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 28724-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28724-1-AP (KCC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 28724-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28724-1-AP (KCC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 28724-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28724-1-AP (KCC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 28724-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28724-1-AP (KCC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse cerebellum using 28724-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using KCC2/SLC12A5 antibody (28724-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse cerebellum using 28724-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using KCC2/SLC12A5 antibody (28724-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: rat brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28724-1-AP targets KCC2/SLC12A5 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KCC2/SLC12A5 fusion protein Ag30203
• Full Name: solute carrier family 12 (potassium-chloride transporter), member 5
• Observed Molecular Weight: 130-150 kDa
• GenBank Accession Number: BC132670
• Gene Symbol: SLC12A5
• Gene ID (NCBI): 57468
• RRID: AB_2918195
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KCC2, encoded by SLC12A5, is a neuron-specific K+-Cl- cotransporter that is essential for Cl- homeostasis and inhibitory synaptic transmission in brain. KCC2 is expressed at high levels in neurons throughout the nervous system. KCC2 deletion caused animal death. Alterations of KCC2 have been linked to neuropathic pain, temporal lobe epilepsy, and other diseases.