KCNE1 Polyclonal antibody

KCNE1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15150-1-AP

Synonyms

ISK, JLNS, JLNS2, KCNE1, LQT2/5, LQT5, Minimal potassium channel, MinK

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Validation Data Gallery

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  WB analysis of mouse heart using 15150-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15150-1-AP (KCNE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 15150-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15150-1-AP (KCNE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse heart tissue using 15150-1-AP

  IF result of anti-KCNE1 (15150-1-AP) in frozen mouse heart section by Dr Nicola Smart.

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse testis tissue
• Positive IF detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

15150-1-AP targets KCNE1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KCNE1 fusion protein Ag3495
• Full Name: potassium voltage-gated channel, Isk-related family, member 1
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 18-25 kDa, 28-35 kDa
• GenBank Accession Number: BC036452
• Gene Symbol: KCNE1
• Gene ID (NCBI): 3753
• RRID: AB_2131977
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
pig	WB	Br J Pharmacol; Aldosterone downregulates slowly activated delayed rectifier potassium current in adult guinea pig cardiomyocytes.; Authors - Yankun Lv
rat	WB	Cardiovasc Ther; LCZ696 Therapy Reduces Ventricular Tachyarrhythmia Inducibility in a Myocardial Infarction-Induced Heart Failure Rat Model.; Authors - Po-Cheng Chang