KIF20B Polyclonal antibody

KIF20B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27269-1-AP

Synonyms

Cancer/testis antigen 90, CT90, KIF20B, kinesin family member 20B, Kinesin like protein KIF20B, KRMP1, M phase phosphoprotein 1, MPHOSPH1, MPP 1, MPP1

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Validation Data Gallery

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  WB analysis using 27269-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27269-1-AP (KIF20B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27269-1-AP

  Mouse Brain tissue lysates were subjected to SDS PAGE followed by western blot with 27269-1-AP (KIF20B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 27269-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27269-1-AP (KIF20B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 27269-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27269-1-AP (KIF20B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 27269-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27269-1-AP (KIF20B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 27269-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27269-1-AP (KIF20B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HT-29 cells, mouse brain tissue, HepG2 cells, Jurkat cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27269-1-AP is KIF20B Fusion Protein expressed in E. coli.

• Tested Reactivity: human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIF20B fusion protein Ag26191
• Full Name: kinesin family member 20B
• Calculated Molecular Weight: 211 kDa
• Observed Molecular Weight: 214 kDa
• GenBank Accession Number: NM_001284259
• Gene Symbol: KIF20B
• Gene ID (NCBI): 9585
• RRID: AB_2918121
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.