KIFC2 Polyclonal antibody

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  WB analysis of mouse brain using 27470-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27470-1-AP (KIFC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 27470-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using KIFC2 antibody (27470-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

KIFC2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 27470-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

kinesin family member C2, Kinesin like protein KIFC2, Kinesin-like protein KIFC2

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27470-1-AP targets KIFC2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIFC2 fusion protein Ag26519
• Full Name: kinesin family member C2
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 96 kDa
• GenBank Accession Number: BC017311
• Gene Symbol: KIFC2
• Gene ID (NCBI): 90990
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96AC6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kinesin family member C2 (KIFC2), one of the kinesin-14 motor family members, is especially expressed in neurons and is important for organizing dendritic microtubules and to control dendrite development (PMID: 37716704). KIFC2 is a microtubule-binding protein that functions as a motor protein in vesicle transport along axons and/or dendrites. Western blot analysis detected KIFC2 at an apparent molecular mass of 96 kD(PMID: 9115737).