CoraLite® Plus 488-conjugated KLRG1 Recombinant antibody

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  IF Staining of Jurkat using CL488-84785-5

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 KLRG1 antibody (CL488-84785-5, Clone: 242327A4 ) at dilution of 1:200.

KLRG1 Uni-rAb^TM Recombinant Antibody for IF/ICC

Cat No. CL488-84785-5

Clone No.242327A4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

242327A4, CLEC15A, C-type lectin domain family 15 member A, ITIM-containing receptor MAFA-L, Killer cell lectin-like receptor subfamily G member 1

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Tested Applications

• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84785-5 targets KLRG1 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KLRG1 fusion protein Eg2036
• Full Name: killer cell lectin-like receptor subfamily G, member 1
• Calculated Molecular Weight: 22 kDa
• GenBank Accession Number: NM_005810.4
• Gene Symbol: KLRG1
• Gene ID (NCBI): 10219
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96E93-1
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

KLRG1 (killer cell lectin-like receptor subfamily G, member 1) is a C-type lectin inhibitory receptor that contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain. KLRG1 is expressed by subsets of NK and T cells, existing both as a monomer and as a disulfide-linked homodimer (PMID: 10741410; 19604491). It is considered to be a cell differentiation marker for NK and T cells and is strongly induced by viral and other infections (PMID: 20373518). Through interactions with members of the cadherin family, KLRG1 plays an inhibitory role on NK- and T-cell function (PMID: 19009530; 20373518).