Cytokeratin 17-Specific Polyclonal antibody

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  WB analysis using 17516-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17516-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 17516-1-AP

  IP result of anti-Cytokeratin 17-Specific (IP:17516-1-AP, 4ug; Detection:17516-1-AP 1:1000) with A431 cells lysate 1200ug.

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  IHC staining of human bowen disease using 17516-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human bowen disease using 17516-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung squamous cell carcinoma using 17516-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung squamous cell carcinoma using 17516-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 17516-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 17516-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 17516-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cytokeratin 17-Specific antibody (17516-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 17516-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 17-Specific antibody (17516-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

Cytokeratin 17-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 17516-1-AP

Publications(28)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Cytokeratin 17, KRT17, CK 17, CK-17, Cytokeratin,Keratin

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, HaCaT cells, mouse skin tissue, rat skin tissue
• Positive IP detected in: A431 cells
• Positive IHC detected in: human bowen disease, human bowen disease tissue, human cervical cancer tissue, human lung squamous cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:20000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:4000-1:16000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 12 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below

Product Information

17516-1-AP targets Cytokeratin 17-Specific in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: keratin 17
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: NM_000422
• Gene Symbol: Cytokeratin 17
• Gene ID (NCBI): 3872
• RRID: AB_2133036
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q04695
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma. This antibody is specifically against KRT17.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse		Science; Spatiotemporal immune zonation of the human kidney.; Authors - Benjamin J Stewart
human	IHC	Clin Cancer Res; A Preclinical Trial and Molecularly Annotated Patient Cohort Identify Predictive Biomarkers in Homologous Recombination-deficient Pancreatic Cancer.; Authors - Yifan Wang
human	WB	Breast Cancer Res; Anillin regulates breast cancer cell migration, growth, and metastasis by non-canonical mechanisms involving control of cell stemness and differentiation.; Authors - Dongdong Wang
human	WB	Oncogene; The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer; Authors - Mengjie Li ; KD Validated
mouse	WB	Phytother Res; Epigallocatechin-3-gallate promotes wound healing response in diabetic mice by activating keratinocytes and promoting re-epithelialization; Authors - Yongling Ning
mouse,human	WB	J Invest Dermatol; Keratin 17 Impacts Global Gene Expression and Controls G2/M Cell Cycle Transition in Ionizing Radiation-Induced Skin Damage; Authors - ZhiCao Yue

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lindsey (Verified Customer) (08-08-2025)

This antibody works very well on both FFPE tissue (with heat based citrate buffer antigen retrieval) and whole mount embryonic skin. It is highly specific based on our experience and can be used from 1/1000 to 1/1200 dilution (1/1000 for adult and 1/1200 for embryonic).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Mouse skin