Cytokeratin 4 Polyclonal antibody

Cytokeratin 4 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 16572-1-AP

Synonyms

CK 4, CK4, CYK4, Cytokeratin 4, K4, keratin 4, KRT4, Type II keratin Kb4



Tested Applications

Positive WB detected inA431 cells, human bladder tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16572-1-AP targets Cytokeratin 4 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 4 fusion protein Ag9854
Full Name keratin 4
Calculated molecular weight 534 aa, 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC042174
Gene symbol KRT4
Gene ID (NCBI) 3851
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 4 is a type II cytokeratins. It is specifically found in differentiated layers of the mucosal and esophageal epithelia together with keratin 13.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 4 antibody 16572-1-APDownload protocol
IHC protocol for Cytokeratin 4 antibody 16572-1-APDownload protocol
IF protocol for Cytokeratin 4 antibody 16572-1-APDownload protocol
FC protocol for Cytokeratin 4 antibody 16572-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Rep

An Immunocompetent Mouse Model of HPV16(+) Head and Neck Squamous Cell Carcinoma.

Authors - Miranda B Carper
humanWB

Oncotarget

Significance of the NOR1-FOXA1/HDAC2-Slug regulatory network in epithelial-mesenchymal transition of tumor cells.

Authors - Wei Wang
humanIHC

Ann Diagn Pathol

The molecular-based differentiation of Heck's disease from its mimics including oral condyloma and white sponge nevus.

Authors - Ziv Schwartz
IF

Development

Novel dynamics of human mucociliary differentiation revealed by single-cell RNA sequencing of nasal epithelial cultures.

Authors - Sandra Ruiz García
mouseIF

Nature

A single-cell atlas of the airway epithelium reveals the CFTR-rich pulmonary ionocyte.

Authors - Lindsey W Plasschaert