Cytokeratin 4 Polyclonal antibody

Cytokeratin 4 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 16572-1-AP

Synonyms

KRT4, Cytokeratin-4, Cytokeratin,Keratin, CYK4, CK-4

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Validation Data Gallery

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  WB analysis of A431 using 16572-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16572-1-AP (Cytokeratin 4 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human bladder using 16572-1-AP

  human bladder tissue were subjected to SDS PAGE followed by western blot with 16572-1-AP (Cytokeratin 4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 16572-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16572-1-AP (Cytokeratin 4 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 16572-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16572-1-AP (Cytokeratin 4 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse skin using 16572-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using Cytokeratin 4 antibody (16572-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 16572-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 4 antibody (16572-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

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Tested Applications

• Positive WB detected in: A431 cells, human bladder tissue
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse skin tissue
• Positive IF/ICC detected in: A431 cells, mouse skin tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Product Information

16572-1-AP targets Cytokeratin 4 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytokeratin 4 fusion protein Ag9854
• Full Name: keratin 4
• Calculated Molecular Weight: 534 aa, 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC042174
• Gene Symbol: Cytokeratin 4
• Gene ID (NCBI): 3851
• RRID: AB_2134041
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 4 is a type II cytokeratins. It is specifically found in differentiated layers of the mucosal and esophageal epithelia together with keratin 13.

Publications

Species	Application	Title
mouse	IF	Nature; A single-cell atlas of the airway epithelium reveals the CFTR-rich pulmonary ionocyte.; Authors - Lindsey W Plasschaert
	IF	Development; Novel dynamics of human mucociliary differentiation revealed by single-cell RNA sequencing of nasal epithelial cultures.; Authors - Sandra Ruiz García
human	IHC	Int J Mol Sci; Early Clinical Outcomes of the First Commercialized Human Autologous Ex Vivo Cultivated Oral Mucosal Epithelial Cell Transplantation for Limbal Stem Cell Deficiency: Two Case Reports and Literature Review; Authors - Hiroshi Toshida
human	IHC	Ann Diagn Pathol; The molecular-based differentiation of Heck's disease from its mimics including oral condyloma and white sponge nevus.; Authors - Ziv Schwartz
mouse	IF	Cell Rep; An Immunocompetent Mouse Model of HPV16(+) Head and Neck Squamous Cell Carcinoma.; Authors - Miranda B Carper
human	WB	Oncotarget; Significance of the NOR1-FOXA1/HDAC2-Slug regulatory network in epithelial-mesenchymal transition of tumor cells.; Authors - Wei Wang