Validation Data Gallery
|Positive WB detected in||A431 cells, human bladder tissue|
|Positive IHC detected in||human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A431 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16572-1-AP targets Cytokeratin 4 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cytokeratin 4 fusion protein Ag9854|
|Full Name||keratin 4|
|Calculated molecular weight||534 aa, 57 kDa|
|Observed molecular weight||57 kDa|
|GenBank accession number||BC042174|
|Gene ID (NCBI)||3851|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 4 is a type II cytokeratins. It is specifically found in differentiated layers of the mucosal and esophageal epithelia together with keratin 13.
|Product Specific Protocols|
|WB protocol for Cytokeratin 4 antibody 16572-1-AP||Download protocol|
|IHC protocol for Cytokeratin 4 antibody 16572-1-AP||Download protocol|
|IF protocol for Cytokeratin 4 antibody 16572-1-AP||Download protocol|
|FC protocol for Cytokeratin 4 antibody 16572-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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