Ki-67 Recombinant monoclonal antibody, PBS Only

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• Ki-67 Recombinant monoclonal antibody, PBS Only (84192-4-PBS)

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  WB analysis using 84192-4-RR (same clone as 84192-4-PBS)

  Various lysates were subjected to SDS page followed by western blot with 84192-4-RR (Ki-67 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

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  IHC staining of human tonsil using 84192-4-RR (same clone as 84192-4-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Tonsil Tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:1000 (under 40x lens).Heat mediated antigen retrieval with Tris EDTA (pH 9.0) buffer. Staining was performed on the Biocare NeoPATH Pro. This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsil using 84192-4-RR (same clone as 84192-4-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Tonsil Tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris EDTA (pH 9.0) buffer. Staining was performed on the Biocare NeoPATH Pro. This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84192-4-RR (same clone as 84192-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84192-4-RR (Ki67 rabbit recombinant antibody) at dilution of 1:8000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb™ Enhanced Anti-Rabbit/Mouse Universal Secondary System Plus RGAU015 was used for detection. This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84192-4-RR (same clone as 84192-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84192-4-RR (same clone as 84192-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 84192-4-RR (same clone as 84192-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 84192-4-RR (same clone as 84192-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

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  IHC staining of Human Spleen using 84192-4-RR (same clone as 84192-4-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Spleen tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

• 

  IHC staining of Human Spleen using 84192-4-RR (same clone as 84192-4-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Spleen tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

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  IHC staining of Human renal cancer using 84192-4-RR (same clone as 84192-4-PBS)

  Immunohistochemical analysis of paraffin-embedded Human renal cancer slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

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  IHC staining of Human renal cancer using 84192-4-RR (same clone as 84192-4-PBS)

  Immunohistochemical analysis of paraffin-embedded Human renal cancer slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human malignant melanoma using 84192-4-RR (same clone as 84192-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human malignant melanoma using 84192-4-RR (same clone as 84192-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 84192-4-RR (same clone as 84192-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 84192-4-RR (same clone as 84192-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

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  IHC staining of human Breast cancer using 84192-4-RR (same clone as 84192-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human Breast cancer tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human Breast cancer using 84192-4-RR (same clone as 84192-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human Breast cancer tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

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  IF Staining of human lung cancer using 84192-4-RR (same clone as 84192-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1/CD54 antibody (60299-1-Ig, Clone: 2F9A8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

• 

  IF Staining of human lung cancer using 84192-4-RR (same clone as 84192-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

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  IF Staining of human thyroid cancer using 84192-4-RR (same clone as 84192-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human thyroid cancer tissue using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1/CD54 antibody (60299-1-Ig, Clone: 2F9A8, red).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

• 

  IF Staining of human thyroid cancer using 84192-4-RR (same clone as 84192-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human thyroid cancer tissue using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1/CD54 antibody (60299-1-Ig, Clone: 2F9A8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84192-4-RR (same clone as 84192-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red) (Cat No. PF00003). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 84192-4-RR (same clone as 84192-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 84192-4-RR (same clone as 84192-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 84192-4-RR (same clone as 84192-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red) (Cat No. PF00003). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 84192-4-RR (same clone as 84192-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red) (Cat No. PF00003). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84192-4-RR (same clone as 84192-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red) (Cat No. PF00003). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 84192-4-RR (same clone as 84192-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red) (Cat No. PF00003). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 84192-4-RR (same clone as 84192-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red) (Cat No. PF00003). This data was developed using the same antibody clone with 84192-4-PBS in a different storage buffer formulation.

Ki67 antibodies are widely used as markers for cell proliferation, enabling the detection and quantification of actively dividing cells in tissue samples for research and diagnostic applications, especially in cancer pathology. Proteintech has an extensive range of Ki-67 antibodies validated for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA, Cell treatment and more.

Cat No. 84192-4-PBS

Clone No.241499E7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

KI67, MKI67, 241499E7, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67

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Product Information

84192-4-PBS targets Ki-67 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: antigen identified by monoclonal antibody Ki-67
• Calculated Molecular Weight: 359 kDa
• Observed Molecular Weight: 359 kDa
• GenBank Accession Number: NM_002417
• Gene Symbol: KI67
• Gene ID (NCBI): 4288
• RRID: AB_3671744
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P46013
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.