LAIR1 Monoclonal antibody

LAIR1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1A5D1

Cat no : 67220-1-Ig

Synonyms

CD305, hLAIR1, LAIR 1, LAIR1



Tested Applications

Positive WB detected inTHP-1 cells, HL-60 cells, TF-1 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67220-1-Ig targets LAIR1 in WB, IHC, IF,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen LAIR1 fusion protein Ag14459
Full Name leukocyte-associated immunoglobulin-like receptor 1
Calculated molecular weight 287 aa, 31 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC027899
Gene symbol LAIR1
Gene ID (NCBI) 3903
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Leukocyte-associated immunoglobulin-like receptor 1 (LAIR1) is a transmembrane glycoprotein with a single immunoglobulin-like domain and a cytoplasmic tail containing two immune receptor tyrosine-based inhibitory motifs (PMID: 9285412). LAIR1 is expressed on the majority of human PBMCs, including NK, T, B, monocytes, and dendritic cells, as well as the majority of thymocytes (PMID: 10229813). It functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of NK cells, B cells and T cells. LAIR1 is a collagen receptor (PMID: 16754721). Tumor-expressed collagens can modulate immune cell function through the inhibitory collagen receptor LAIR1 (PMID: 21955987).

Protocols

Product Specific Protocols
WB protocol for LAIR1 antibody 67220-1-IgDownload protocol
IHC protocol for LAIR1 antibody 67220-1-IgDownload protocol
IF protocol for LAIR1 antibody 67220-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols