LCE1B Polyclonal antibody

LCE1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21771-1-AP

Synonyms

LEP2, SPRL2A, Small proline-rich-like epidermal differentiation complex protein 2A, Late envelope protein 2, late cornified envelope 1B

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Validation Data Gallery

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  WB analysis of A375 using 21771-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 21771-1-AP (LCE1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 21771-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 21771-1-AP (LCE1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skin using 21771-1-AP

  rat skin tissue were subjected to SDS PAGE followed by western blot with 21771-1-AP (LCE1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 21771-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 21771-1-AP (LCE1B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 21771-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 21771-1-AP (LCE1B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human oesophagus using 21771-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 21771-1-AP (LCE1B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human oesophagus using 21771-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 21771-1-AP (LCE1B antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A375 using 21771-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using LCE1B antibody (21771-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: A375 cells, mouse skin tissue, rat skin tissue
• Positive IHC detected in: human skin tissue, human oesophagus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21771-1-AP targets LCE1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LCE1B fusion protein Ag16442
• Full Name: late cornified envelope 1B
• Calculated Molecular Weight: 118 aa, 12 kDa
• Observed Molecular Weight: 10-12 kDa
• GenBank Accession Number: BC104232
• Gene Symbol: LCE1B
• Gene ID (NCBI): 353132
• RRID: AB_10858482
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human LCE1B (Late cornified envelope protein 1B), also named late envelope protein 2 (LEP2), is located at human Chromosome1q21 which contains multiple LCE clusters. LCE1B gene is predicated to encode a 118-aa protein, and is currently evidenced at transcript level. Its expression was skin-specific and was readily detected in adult trunk skin, adult arm skin, fetal skin, penal skin, vulva, esophagus and tongue (PMID: 15854049). LCE genes respond to environmental stimuli such as calcium levels and ultraviolet (UV) light, however, LCE groups have distinct functions (PMID: 15854049).