LCK Monoclonal antibody, PBS Only (Capture)

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  WB analysis of pig thymus using 60162-1-Ig (same clone as 60162-1-PBS)

  pig thymus tissue were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

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  WB analysis using 60162-1-Ig (same clone as 60162-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

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  WB analysis of rat thymus using 60162-1-Ig (same clone as 60162-1-PBS)

  rat thymus tissue were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 60162-1-Ig (same clone as 60162-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

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  WB analysis of Ramos using 60162-1-Ig (same clone as 60162-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60162-1-Ig (same clone as 60162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60162-1-Ig (same clone as 60162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 60162-1-Ig (same clone as 60162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 60162-1-Ig (same clone as 60162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 60162-1-Ig (same clone as 60162-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 60162-1-Ig (same clone as 60162-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60162-1-Ig (same clone as 60162-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60162-1-Ig (same clone as 60162-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 60162-1-Ig (same clone as 60162-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human LCK (60162-1-Ig, Clone:2D10C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 60162-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51238-1

  Cytometric bead array standard curve of MP51238-1, LCK Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60162-1-PBS. Detection antibody: 60162-3-PBS. Standard:Ag7694. Range: 0.098-25 ng/mL.

LCK Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 60162-1-PBS

Clone No.2D10C2

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

2D10C2, EC:2.7.10.2, Lck p56, Leukocyte C-terminal Src kinase, LSK

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Product Information

60162-1-PBS targets LCK as part of a matched antibody pair:

MP51238-1: 60162-1-PBS capture and 60162-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7694

  Product name: Recombinant human LCK protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 236-539 aa of BC013200

  Sequence: DEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHQRLVRLYAVVTQEPIYIITEYMENDTLLDSQLEEKGLGASPWGNLGQQLLLLPTGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSDTLSCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYQLMRLCWKERPEDRPTFDYLRSVLEDFFTATEGQYQPQP

• Full Name: lymphocyte-specific protein tyrosine kinase
• Calculated Molecular Weight: 539 aa, 56 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC013200
• Gene Symbol: LCK
• Gene ID (NCBI): 3932
• RRID: AB_10663101
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P06239
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.