Tested Applications
| Positive WB detected in | NK-92 cells, Jurkat cells, pig thymus tissue, Ramos cells, rat thymus tissue, MOLT-4 cells, MJ cells |
| Positive IHC detected in | human tonsillitis tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60162-1-Ig targets LCK in WB, IHC, IF/ICC, IF-P, FC (Intra), CoIP, ELISA applications and shows reactivity with human, rat, pig samples.
| Tested Reactivity | human, rat, pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7694 Product name: Recombinant human LCK protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 236-539 aa of BC013200 Sequence: DEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHQRLVRLYAVVTQEPIYIITEYMENDTLLDSQLEEKGLGASPWGNLGQQLLLLPTGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSDTLSCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYQLMRLCWKERPEDRPTFDYLRSVLEDFFTATEGQYQPQP Predict reactive species |
| Full Name | lymphocyte-specific protein tyrosine kinase |
| Calculated Molecular Weight | 539 aa, 56 kDa |
| Observed Molecular Weight | 50-60 kDa |
| GenBank Accession Number | BC013200 |
| Gene Symbol | LCK |
| Gene ID (NCBI) | 3932 |
| RRID | AB_10663101 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P06239 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for LCK antibody 60162-1-Ig | Download protocol |
| IF protocol for LCK antibody 60162-1-Ig | Download protocol |
| IHC protocol for LCK antibody 60162-1-Ig | Download protocol |
| WB protocol for LCK antibody 60162-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LCK Monoclonal antibody (2D10C2), catalog number 60162-1-Ig, has 8 citations. These appear in journals including Cancer Letters, Molecular Medicine, Journal of Medicinal Chemistry, Ecotoxicology and Environmental Safety, Frontiers in Pharmacology, BMC Cancer, International Journal of General Medicine, and Computer Methods and Biomechanics: Biomedical Engineering. Research fields span colorectal cancer immunophenotyping, asthma immunology, drug discovery, ischemic stroke, anti-inflammatory mechanisms, glioma prognosis, oral and laryngeal squamous cell carcinoma, and tumor microenvironment analysis.
| Species | Application | Title |
|---|---|---|
Cancer Lett Distinct Immunophenotypic Profiles and Neutrophil Heterogeneity in Colorectal Cancer | ||
Mol Med c-CBL/LCK/c-JUN/ETS1/CD28 axis restrains childhood asthma by suppressing Th2 differentiation | ||
J Med Chem Discovery of Phosphorylated Peptidomimetics Targeting Cbl-b SH2 Domain as Orthosteric Cbl-b Inhibitors by an Optimized Fluorescence Polarization Competition Assay | ||
Ecotoxicol Environ Saf HFPO-DA exacerbates acute ischemic stroke outcomes in rats via the CSNK2A1/GSK3B/NF-κB signaling pathway. | ||
Front Pharmacol Study on the anti-inflammatory effects and mechanisms of gentisic acid based on the LPS-induced RAW264.7 cell inflammation model and the oxazolone-induced zebrafish inflammation model. | ||
BMC Cancer Prognostic value of LPAR1 expression and methylation in low-grade gliomas: a meta-analysis of TCGA and CGGA datasets and functional validation. |
