LCK Monoclonal antibody

LCK Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2D10C2

Cat no : 60162-1-Ig

Synonyms

LCK, LSK, p56 LCK, p56lck, pp58lck, Protein YT16, Proto oncogene Lck, Tyrosine protein kinase Lck, YT16



Tested Applications

Positive WB detected inpig thymus tissue, Ramos cells, rat thymus tissue
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

60162-1-Ig targets LCK in WB, IHC, IF,ELISA applications and shows reactivity with human, pig, rat samples.

Tested Reactivity human, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LCK fusion protein Ag7694
Full Name lymphocyte-specific protein tyrosine kinase
Calculated molecular weight 539 aa, 56 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC013200
Gene symbol LCK
Gene ID (NCBI) 3932
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for LCK antibody 60162-1-IgDownload protocol
IHC protocol for LCK antibody 60162-1-IgDownload protocol
IF protocol for LCK antibody 60162-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols