pig thymus tissue were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
pig thymus tissue were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 60162-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 60162-1-Ig
Jurkat cells were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Jurkat cells were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of Ramos using 60162-1-Ig
Ramos cells were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Ramos cells were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of rat thymus using 60162-1-Ig
rat thymus tissue were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
rat thymus tissue were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human tonsillitis using 60162-1-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 60162-1-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 60162-1-Ig
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 60162-1-Ig
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human tonsillitis using 60162-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of human tonsillitis using 60162-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of HepG2 using 60162-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).
FC experiment of HepG2 using 60162-1-Ig
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human LCK (60162-1-Ig, Clone:2D10C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human LCK (60162-1-Ig, Clone:2D10C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human tonsillitis tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
human tonsillitis tissue
Positive IF/ICC detected in
HepG2 cells
Positive FC (Intra) detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Immunofluorescence (IF)-P
IF-P : 1:200-1:800
Immunofluorescence (IF)/ICC
IF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.
pig thymus tissue were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 60162-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 60162-1-Ig
Jurkat cells were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of Ramos using 60162-1-Ig
Ramos cells were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of rat thymus using 60162-1-Ig
rat thymus tissue were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human tonsillitis using 60162-1-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 60162-1-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 60162-1-Ig
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 60162-1-Ig
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P Figures
IF Staining of human tonsillitis using 60162-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of human tonsillitis using 60162-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF/ICC Figures
IF Staining of HepG2 using 60162-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).
IF Staining of HepG2 using 60162-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).
FC (INTRA) Figures
FC experiment of HepG2 using 60162-1-Ig
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human LCK (60162-1-Ig, Clone:2D10C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
LCK Monoclonal antibody
Catalog Number
60162-1-Ig
Citations
2
Dilutions
WB : 1:5000-1:50000 IHC : 1:500-1:2000 IF-P : 1:200-1:800 IF/ICC : 1:400-1:1600 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Reactivity
human, rat, pig
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
