|Positive WB detected in||HEK-293 cells, MCF-7 cells, human testis tissue, A375 cells, HepG2 cells, NIH/3T3 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:30000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21799-1-AP is LDHA Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||LDHA fusion protein Ag16703|
|Full Name||lactate dehydrogenase A|
|Calculated molecular weight||332 aa, 37 kDa|
|Observed molecular weight||32-37 kDa|
|GenBank accession number||BC067223|
|Gene ID (NCBI)||3939|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Lactate dehydrogenase (LDH) is composed of A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1)genes, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. It has some isoforms with the molecular mass of 27-40 kDa and can form a homotetramer(PMID:11276087).
|Product Specific Protocols|
|WB protocol for LDHA antibody 21799-1-AP||Download protocol|
|IHC protocol for LDHA antibody 21799-1-AP||Download protocol|
|IF protocol for LDHA antibody 21799-1-AP||Download protocol|
|IP protocol for LDHA antibody 21799-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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