LDHA Polyclonal antibody

LDHA Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 21799-1-AP

Synonyms

lactate dehydrogenase A, LDH A, LDH M, LDH muscle subunit, LDH1, LDHA, PIG19



Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, human testis tissue, A375 cells, HepG2 cells, NIH/3T3 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:30000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21799-1-AP is LDHA Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LDHA fusion protein Ag16703
Full Name lactate dehydrogenase A
Calculated molecular weight 332 aa, 37 kDa
Observed molecular weight 32-37 kDa
GenBank accession numberBC067223
Gene symbol LDHA
Gene ID (NCBI) 3939
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lactate dehydrogenase (LDH) is composed of A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1)genes, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. It has some isoforms with the molecular mass of 27-40 kDa and can form a homotetramer(PMID:11276087).

Protocols

Product Specific Protocols
WB protocol for LDHA antibody 21799-1-APDownload protocol
IHC protocol for LDHA antibody 21799-1-APDownload protocol
IF protocol for LDHA antibody 21799-1-APDownload protocol
IP protocol for LDHA antibody 21799-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cancer Res

HBXIP and LSD1 Scaffolded by lncRNA Hotair Mediate Transcriptional Activation by c-Myc.

Authors - Yinghui Li
ratWB,IHC

Arch Gynecol Obstet

Hypoxia-induced regulation of placental REDD1 and mTOR was impaired in a rat model of estrogen-induced cholestasis.

Authors - Fan Zhou
humanWB

Placenta

Expression of hypoxia-regulated genes and glycometabolic genes in placenta from patients with intrahepatic cholestasis of pregnancy.

Authors - W Wei
humanWB,IHC

Am J Transl Res

Collagen prolyl 4-hydroxylase 2 predicts worse prognosis and promotes glycolysis in cervical cancer.

Authors - Qingxian Li
humanWB

Life Sci

MiR-223-3p targets FOXO3a to inhibit radiosensitivity in prostate cancer by activating glycolysis.

Authors - Keqin Zhou
ratWB,IHC

Biomed Pharmacother

Protective effects of Weipixiao decoction against MNNG-induced gastric precancerous lesions in rats.

Authors - Tiantian Cai