• Featured Product
  • KD/KO Validated

LGMN Monoclonal antibody

LGMN Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 67017-1-Ig
Clone No.1F2B12

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, mouse and More (1)

Applications

WB, IHC, IF-P, ELISA

AEP, Asparaginyl endopeptidase, legumain, LGMN, LGMN1, Protease, cysteine 1, PRSC1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inSK-BR-3 cells, HEK-293 cells, NCCIT cells, pig kidney tissue, HeLa cells, MCF-7 cells, LNCaP cells, human placenta tissue, JAR cells, Jurkat cells, ATDC-5 cells
Positive IHC detected inhuman kidney tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67017-1-Ig targets LGMN in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, pig, mouse samples.

Tested Reactivity Human, pig, mouse
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7218

Product name: Recombinant human LGMN protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 82-433 aa of BC003061

Sequence: TPGIVINRPNGTDVYQGVPKDYTGEDVTPQNFLAVLRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPNEDLHVKDLNETIHYMYKHKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGDWYSVNWMEDSDVEDLTKETLHKQYHLVKSHTNTSHVMQYGNKTISTMKVMQFQGMKRKASSPVPLPPVTHLDLTPSPDVPLTIMKRKLMNTNDLEESRQLTEEIQRHLDARHLIEKSVRKIVSLLAASEAEVEQLLSERAPLTGHSCYPEALLHFRTHCFNWHSPTYEYALRHLYVLVNLCEKPYPLHRIKLSMDHVCLGHY

Predict reactive species
Full Name legumain
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC003061
Gene Symbol LGMN
Gene ID (NCBI) 5641
RRIDAB_2882333
Conjugate Unconjugated
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ99538
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LGMN (Legumain), also known as asparaginyl endopeptidase or AEP, is a broadly expressed lysosomal cysteine endopeptidase that belongs to peptidase family C13 and specifcally hydrolyzes substrate asparaginyl bonds (PMID: 9065484). LGMN directly regulates diverse physiological and pathological processes by remodeling tissue-specific targets (PMID: 25205715, 21292981, 18820679). In addition, LGMN indirectly contributes to atherosclerotic plaque instability through activation of cathepsin L in the arterial ECM (PMID: 19671471, 18377911). LGMN is secreted as inactive prolegumain (56 kDa) and processed into enzymatically active 46 and 36 kDa forms, as well as a 17 kDa enzymatically inactive C-terminal fragment (PMID: 28162997, PMID: 19671471).

Protocols

Product Specific Protocols
WB protocol for LGMN antibody 67017-1-IgDownload protocol
IHC protocol for LGMN antibody 67017-1-IgDownload protocol
IF protocol for LGMN antibody 67017-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Control Release

A "dual-guide" bioinspired drug delivery strategy of a macrophage-based carrier against postoperative triple-negative breast cancer recurrence.

Authors - Yue Qiu
WB

Front Microbiol

A replicative recombinant HPV16 E7 expression virus upregulates CD36 in C33A cells

Authors - Yunting Shao
mouseWB

Int J Pharm

Macrophage-mediated multi-mode drug release system for photothermal combined with anti-inflammatory therapy against postoperative recurrence of triple negative breast cancer.

Authors - Kebai Ren
humanWB

Int J Biol Sci

Legumain Promotes Gastric Cancer Progression Through Tumor-associated Macrophages In vitro and In vivo.

Authors - Hongbin Wang
humanWB

J Transl Med

The CD163 + tissue-infiltrating macrophages regulate ferroptosis in thyroid-associated ophthalmopathy orbital fibroblasts via the TGF-β/Smad2/3 signaling pathway

Authors - Xuemei Li
humanWB,IHC

Exp Eye Res

Legumain affects the PI3K/AKT tumor progression pathway in retinoblastoma

Authors - Qin Tang
  • KD Validated
Loading...
||
New chat Able
正在加载,请稍候...