LGMN Monoclonal antibody, PBS Only

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  WB analysis using 67017-1-Ig (same clone as 67017-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67017-1-Ig (same clone as 67017-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67017-1-Ig (same clone as 67017-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 67017-1-Ig (same clone as 67017-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 67017-1-Ig (same clone as 67017-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig kidney using 67017-1-Ig (same clone as 67017-1-PBS)

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67017-1-Ig (same clone as 67017-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 67017-1-Ig (same clone as 67017-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig kidney using 67017-1-Ig (same clone as 67017-1-PBS)

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67017-1-Ig (LGMN antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67017-1-Ig (LGMN antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 67017-1-Ig (same clone as 67017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67017-1-Ig (LGMN antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 67017-1-Ig (same clone as 67017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67017-1-Ig (LGMN antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

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  IF Staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using LGMN antibody (67017-1-Ig, Clone: 1F2B12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using LGMN antibody (67017-1-Ig, Clone: 1F2B12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

LGMN Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 67017-1-PBS

Clone No.1F2B12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

PRSC1, legumain, EC:3.4.22.34, 1F2B12

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Product Information

67017-1-PBS targets LGMN in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7218

  Product name: Recombinant human LGMN protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 82-433 aa of BC003061

  Sequence: TPGIVINRPNGTDVYQGVPKDYTGEDVTPQNFLAVLRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPNEDLHVKDLNETIHYMYKHKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGDWYSVNWMEDSDVEDLTKETLHKQYHLVKSHTNTSHVMQYGNKTISTMKVMQFQGMKRKASSPVPLPPVTHLDLTPSPDVPLTIMKRKLMNTNDLEESRQLTEEIQRHLDARHLIEKSVRKIVSLLAASEAEVEQLLSERAPLTGHSCYPEALLHFRTHCFNWHSPTYEYALRHLYVLVNLCEKPYPLHRIKLSMDHVCLGHY

• Full Name: legumain
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC003061
• Gene Symbol: LGMN
• Gene ID (NCBI): 5641
• RRID: AB_2882333
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q99538
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

LGMN (Legumain), also known as asparaginyl endopeptidase or AEP, is a broadly expressed lysosomal cysteine endopeptidase that belongs to peptidase family C13 and specifcally hydrolyzes substrate asparaginyl bonds (PMID: 9065484). LGMN directly regulates diverse physiological and pathological processes by remodeling tissue-specific targets (PMID: 25205715, 21292981, 18820679). In addition, LGMN indirectly contributes to atherosclerotic plaque instability through activation of cathepsin L in the arterial ECM (PMID: 19671471, 18377911). LGMN is secreted as inactive prolegumain (56 kDa) and processed into enzymatically active 46 and 36 kDa forms, as well as a 17 kDa enzymatically inactive C-terminal fragment (PMID: 28162997, PMID: 19671471).