Anti-Human LIGHT/CD258 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98063-1-RR

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were stained with 0.25 ug Anti-Human LIGHT/CD258 Rabbit Recombinant Antibody (98063-1-RR, Clone:240694D7) and PE-Conjugated Goat Anti-Rabbit IgG (H+L). Cells were then stained with APC Anti-Human CD8a. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98063-1-RR

  1x10^6 untreated (black) or PMA, Ionomycin and Brefeldin A treated (red) human PBMCs were stained with 0.25 ug Anti-Human LIGHT/CD258 Rabbit Recombinant Antibody (98063-1-RR, Clone:240694D7) and PE-Conjugated Goat Anti-Rabbit IgG (H+L), or 0.25 ug Isotype Control (blue). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98063-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98063-1-RR against Human LIGHT/CD258 were performed. The affinity constant is 1.25 nM.

LIGHT/CD258 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98063-1-RR

Clone No.240694D7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

LIGHT, HVEM-L, HVEML, HVEM L, CD258

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Tested Applications

• Positive FC detected in: PMA, Ionomycin and Brefeldin A treated human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98063-1-RR targets LIGHT/CD258 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Fusion Protein

• Full Name: tumor necrosis factor (ligand) superfamily, member 14
• Calculated Molecular Weight: 26KD
• GenBank Accession Number: NM_003807
• Gene Symbol: LIGHT
• Gene ID (NCBI): 8740
• RRID: AB_3672210
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O43557
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

LIGHT, also known as TNFSF14, HVEML, or CD258, is a type II transmembrane protein that belongs to the TNF superfamily (PMID: 9462508). It is predominantly expressed in lymphoid tissues and produced by activated T cells and immature dendritic cells (DCs) (PMID: 9462508; 10754304). Through its two primary functional receptors HVEM (TNFRSF14) and lymphotoxin β receptor (LTβR), LIGHT signaling is involved in development and maintenance of lymphoid tissues, as well as innate and adaptive immune responses (PMID: 24575096; 26720335). In addition to the membrane form, LIGHT can also exist as a soluble form generated by cleavage of the extracellular portion of the membrane form.