FITC Plus Anti-Human LIGHT/CD258 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using FITC-98063

  1x10^6 untreated or PMA and ionomycin treated human PBMCs were surface stained with PE Anti-Human CD8a and 5 ul FITC Plus Anti-Human LIGHT/CD258 Rabbit RecAb (FITC-98063, Clone: 240694D7). Cells were not fixed.

• 

  FC experiment of human PBMCs using FITC-98063

  1x10^6 PMA, Ionomycin and Brefeldin A treated human PBMCs were surface stained with 5 ul FITC Plus Anti-Human LIGHT/CD258 Rabbit RecAb (FITC-98063, Clone: 240694D7) (red) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul FITC Plus Anti-Human LIGHT/CD258 Rabbit RecAb (FITC-98063, Clone: 240694D7) (black, dashed). Cells were not fixed.

LIGHT/CD258 Uni-rAb^TM Recombinant Antibody for FC

Cat No. FITC-98063

Clone No.240694D7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

LIGHT, 240694D7, CD258, HVEM L, HVEML

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Tested Applications

• Positive FC detected in: PMA and ionomycin treated human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

FITC-98063 targets LIGHT/CD258 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LIGHT/CD258 fusion protein Eg32095
• Full Name: tumor necrosis factor (ligand) superfamily, member 14
• Calculated Molecular Weight: 26KD
• GenBank Accession Number: NM_003807
• Gene Symbol: LIGHT
• Gene ID (NCBI): 8740
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43557
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

LIGHT, also known as TNFSF14, HVEML, or CD258, is a type II transmembrane protein that belongs to the TNF superfamily (PMID: 9462508). It is predominantly expressed in lymphoid tissues and produced by activated T cells and immature dendritic cells (DCs) (PMID: 9462508; 10754304). Through its two primary functional receptors HVEM (TNFRSF14) and lymphotoxin β receptor (LTβR), LIGHT signaling is involved in development and maintenance of lymphoid tissues, as well as innate and adaptive immune responses (PMID: 24575096; 26720335). In addition to the membrane form, LIGHT can also exist as a soluble form generated by cleavage of the extracellular portion of the membrane form.