LIMD1 Polyclonal antibody

LIMD1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 28106-1-AP

Synonyms

LIM domains containing 1, LIMD1

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Validation Data Gallery

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  WB analysis using 28106-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28106-1-AP (LIMD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 28106-1-AP

  IP result of anti-LIMD1 (IP:28106-1-AP, 4ug; Detection:28106-1-AP 1:1000) with HeLa cells lysate 2640 ug.

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  IF Staining of HeLa using 28106-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 28106-1-AP (LIMD1 antibody) at dilution of 1:400 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

28106-1-AP targets LIMD1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LIMD1 fusion protein Ag27974
• Full Name: LIM domains containing 1
• Calculated Molecular Weight: 72 aa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: NM_014240
• Gene Symbol: LIMD1
• Gene ID (NCBI): 8994
• RRID: AB_2881062
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIMD1 is a member of the Ajuba family of LIM domain-containing proteins, these kinds of proteins have been shown to play a role in intracellular signaling, transcriptional regulation and cellular differentiation, proliferation and migration (PMID:17174104). LIMD1 predominantly localizes to the cytoplasm especially in the E-cadherin cell-cell adhesive junction, yet also translocates to the nucleus when functions as an RB corepressor (PMID:19060205). And LIMD1 acts as a scaffolding protein to form a PHD-LIMD1-VHL axis, facilitating HIF1α ubiquitination and degradation by the proteasome (PMID:22800800). LIMD1 specifically interacts with retinoblastoma protein (pRB), inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. As a tumor suppressor, LIMD1 blocks tumor growth in vitro and in vivo, and is frequently down-regulated in human lung tumors (PMID:15542589), and it also be detected in normal and breast cancer tissues. LIMD1 protein exists several phosphorylation sites, which may affect its theoretical molecular weight when tested. Proteintech's 28106-1-AP antibody was generated by 133 amino acids, it could recognize the full-length protein in applications.

Publications

Species	Application	Title
human	IF	J Cell Biol; Paxillin phase separation promotes focal adhesion assembly and integrin signaling; Authors - Peigang Liang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Paul (Verified Customer) (11-24-2022)

Clear specific signal just above 70kDa

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Different lung cell lines (A549, H1299)