Tested Applications
| Positive WB detected in | A549 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28106-1-AP targets LIMD1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27974 Product name: Recombinant human LIMD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 128-260 aa of NM_014240 Sequence: MPPQEQRSRPYLHGTRHGSQDCGSRESLATSEMSAFHQPGPCEDPSCLTHGDYYDNLSLASPKWGDKPGVSPSIGLSVGSGWPSSPGSDPPLPKPCGDHPLNHRQLSLSSSRSSEGSLGGQNSGIGGRSSEKPT Predict reactive species |
| Full Name | LIM domains containing 1 |
| Calculated Molecular Weight | 72 aa |
| Observed Molecular Weight | 72 kDa |
| GenBank Accession Number | NM_014240 |
| Gene Symbol | LIMD1 |
| Gene ID (NCBI) | 8994 |
| RRID | AB_2881062 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UGP4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LIMD1 is a member of the Ajuba family of LIM domain-containing proteins, these kinds of proteins have been shown to play a role in intracellular signaling, transcriptional regulation and cellular differentiation, proliferation and migration (PMID:17174104). LIMD1 predominantly localizes to the cytoplasm especially in the E-cadherin cell-cell adhesive junction, yet also translocates to the nucleus when functions as an RB corepressor (PMID:19060205). And LIMD1 acts as a scaffolding protein to form a PHD-LIMD1-VHL axis, facilitating HIF1α ubiquitination and degradation by the proteasome (PMID:22800800). LIMD1 specifically interacts with retinoblastoma protein (pRB), inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. As a tumor suppressor, LIMD1 blocks tumor growth in vitro and in vivo, and is frequently down-regulated in human lung tumors (PMID:15542589), and it also be detected in normal and breast cancer tissues. LIMD1 protein exists several phosphorylation sites, which may affect its theoretical molecular weight when tested. Proteintech's 28106-1-AP antibody was generated by 133 amino acids, it could recognize the full-length protein in applications.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for LIMD1 antibody 28106-1-AP | Download protocol |
| WB protocol for LIMD1 antibody 28106-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LIMD1 Polyclonal antibody (Cat# 28106-1-AP) has 1 citation to date. The cited publication appeared in Journal of Cell Biology (2024) and investigated how Paxillin phase separation drives focal adhesion assembly and integrin signaling. The research field encompasses cell adhesion biology, integrin-mediated signaling, and biomolecular condensates/phase separation in human cells. The antibody was applied in immunofluorescence (IF) for this study.
| Species | Application | Title |
|---|---|---|
J Cell Biol Paxillin phase separation promotes focal adhesion assembly and integrin signaling |
Reviews
What customers say
The product received a 5-star rating for Western Blot. The reviewer used a 1/1000 dilution on different lung cell lines (A549, H1299) and reported a clear, specific signal just above 70 kDa. Overall, the feedback highlights strong specificity and reliable performance in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Paul (Verified Customer) (11-24-2022) | Clear specific signal just above 70kDa
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