LRRC50 Polyclonal antibody

LRRC50 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30156-1-AP

Synonyms

DKFZp434A119, DNAAF1, FLJ25330, LRRC50

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Validation Data Gallery

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  IHC staining of mouse testis using 30156-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30156-1-AP (LRRC50 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 30156-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30156-1-AP (LRRC50 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse testis using 30156-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using LRRC50 antibody (30156-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 30156-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using LRRC50 antibody (30156-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse testis tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30156-1-AP targets LRRC50 in IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LRRC50 fusion protein Ag32822
• Full Name: leucine rich repeat containing 50
• Calculated Molecular Weight: 725 aa, 80 kDa
• GenBank Accession Number: BC024009
• Gene Symbol: LRRC50
• Gene ID (NCBI): 123872
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LRRC50 is a cilium-specific protein required for the stability of the ciliary architecture, it plays a role in cytoplasmic preassembly of dynein arms.