Anti-Mouse Ly-6C (HK1.4)

• 

  FC experiment of mouse bone marrow cells using 65296-1-Ig

  1x10^6 mouse bone marrow cells were surface stained with 0.4 ug Anti-Mouse Ly-6C (HK1.4) (65296-1-Ig, Clone: HK1.4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.4 ug Isotype Control (blue). Cells were not fixed.

Ly-6C Monoclonal Antibody for FC

Cat No. 65296-1-Ig

Clone No.HK1.4

Publications(2)

Host / Isotype

Rat / IgG2c

Reactivity

mouse

Applications

IF, FC

Synonyms

Ly6c, HK1.4, Ly 6C, Ly6c1

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.4 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

65296-1-Ig targets Ly-6C in IF, FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2c
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  L3 cloned CTL cells

• Full Name: lymphocyte antigen 6 complex, locus C1
• GenBank Accession Number: BC010764
• Gene Symbol: Ly-6C
• Gene ID (NCBI): 17067
• RRID: AB_3670287
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P0CW02
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Ly-6C (lymphocyte antigen 6 complex, locus C) is a 14-17 kDa GPI-linked cell surface antigen that belongs to the Ly-6 family of glycosyl phosphatidylinositol-anchored surface glycoproteins (PMID: 9192663). Ly-6C is expressed on mouse monocytes/macrophages, endothelial cells, granulocytes, thymocytes, and some T cell subsets (PMID: 2849552). The two alleles of Ly-6C, Ly-6C.1 and Ly-6C.2, result in differential expression of this protein on subpopulations of CD4+ T cells (PMID: 15057973; 9126982). Ly-6C has been reported as a marker of memory CD8+ T cells (PMID: 7543536).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	J Exp Med; Tet2 deficiency-induced expansion of monocyte-derived macrophages promotes liver fibrosis.; Authors - Jiuxing Feng
mouse	IF	J Leukoc Biol; Galectin-3 promotes sialyl Lewis X epitope biosynthesis in monocytes.; Authors - Jennifer Judge