CoraLite® Plus 647 Anti-Mouse Ly-6C (HK1.4)

• 

  FC experiment of mouse bone marrow cells using CL647-65296

  1x10^6 mouse bone marrow cells were surface stained with FITC Plus Anti-Mouse CD11b (M1/70) (FITC-65055, Clone: M1/70), and 0.25 ug CoraLite® Plus 647 Anti-Mouse Ly-6C (HK1.4) (CL647-65296, Clone: HK1.4) or unstained. Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using CL647-65296

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse Ly-6C (HK1.4) (CL647-65296, Clone: HK1.4) (red), or unstained (blue). Cells were not fixed.

Ly-6C Monoclonal Antibody for FC

Cat No. CL647-65296

Clone No.HK1.4

Host / Isotype

Rat / IgG2c

Reactivity

mouse

Applications

FC

Synonyms

Ly6c, HK1.4, Ly 6C, Ly6c1

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65296 targets Ly-6C in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2c
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  L3 cloned CTL cells

• Full Name: lymphocyte antigen 6 complex, locus C1
• GenBank Accession Number: BC010764
• Gene Symbol: Ly-6C
• Gene ID (NCBI): 17067
• RRID: AB_3673578
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P0CW02
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Ly-6C (lymphocyte antigen 6 complex, locus C) is a 14-17 kDa GPI-linked cell surface antigen that belongs to the Ly-6 family of glycosyl phosphatidylinositol-anchored surface glycoproteins (PMID: 9192663). Ly-6C is expressed on mouse monocytes/macrophages, endothelial cells, granulocytes, thymocytes, and some T cell subsets (PMID: 2849552). The two alleles of Ly-6C, Ly-6C.1 and Ly-6C.2, result in differential expression of this protein on subpopulations of CD4+ T cells (PMID: 15057973; 9126982). Ly-6C has been reported as a marker of memory CD8+ T cells (PMID: 7543536).