MARS Monoclonal antibody

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  WB analysis using 67739-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67739-1-Ig (MARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 67739-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 67739-1-Ig (MARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67739-1-Ig

  WB result of MARS antibody (67739-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MARS transfected HepG2 cells.

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  IF Staining of HepG2 using 67739-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MARS antibody (67739-1-Ig, Clone: 3E3B4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

MARS Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 67739-1-Ig

Clone No.3E3B4

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

MARS1, 3E3B4, EC:6.1.1.10, Methionine--tRNA ligase, cytoplasmic, Methionyl-tRNA synthetase

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, HepG2 cells, Jurkat cells, K-562 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67739-1-Ig targets MARS in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6961

  Product name: Recombinant human MARS protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 552-900 aa of BC002384

  Sequence: MAKDNVPFHSLVFPCSALGAEDNYTLVSHLIATEYLNYEDGKFSKSRGVGVFGDMAQDTGIPADIWRFYLLYIRPEGQDSAFSWTDLLLKNNSELLNNLGNFINRAGMFVSKFFGGYVPEMVLTPDDQRLLAHVTLELQHYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGSEADRQRAGTVTGLAVNIAALLSVMLQPYMPTVSATIQAQLQLPPPACSILLTNFLCTLPAGHQIGTVSPLFQKLENDQIESLRQRFGGGQAKTSPKPAVVETVTTAKPQQIQALMDEVTKQGNIVRELKAQKADKNEVAAEVAKLLDLKKQLAVAEGKPPEAPKGKKKK

• Full Name: methionyl-tRNA synthetase
• Calculated Molecular Weight: 101 kDa
• Observed Molecular Weight: 97-101 kDa
• GenBank Accession Number: BC002384
• Gene Symbol: MARS
• Gene ID (NCBI): 4141
• RRID: AB_2918508
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P56192
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.