MARS Monoclonal antibody, PBS Only

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  WB analysis using 67739-1-Ig (same clone as 67739-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67739-1-Ig (MARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67739-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 67739-1-Ig (same clone as 67739-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 67739-1-Ig (MARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67739-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67739-1-Ig (same clone as 67739-1-PBS)

  WB result of MARS antibody (67739-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MARS transfected HepG2 cells. This data was developed using the same antibody clone with 67739-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67739-1-Ig (same clone as 67739-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MARS antibody (67739-1-Ig, Clone: 3E3B4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67739-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

MARS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 67739-1-PBS

Clone No.3E3B4

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

MARS1, 3E3B4, EC:6.1.1.10, Methionine--tRNA ligase, cytoplasmic, Methionyl-tRNA synthetase

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Product Information

67739-1-PBS targets MARS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6961

  Product name: Recombinant human MARS protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 552-900 aa of BC002384

  Sequence: MAKDNVPFHSLVFPCSALGAEDNYTLVSHLIATEYLNYEDGKFSKSRGVGVFGDMAQDTGIPADIWRFYLLYIRPEGQDSAFSWTDLLLKNNSELLNNLGNFINRAGMFVSKFFGGYVPEMVLTPDDQRLLAHVTLELQHYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGSEADRQRAGTVTGLAVNIAALLSVMLQPYMPTVSATIQAQLQLPPPACSILLTNFLCTLPAGHQIGTVSPLFQKLENDQIESLRQRFGGGQAKTSPKPAVVETVTTAKPQQIQALMDEVTKQGNIVRELKAQKADKNEVAAEVAKLLDLKKQLAVAEGKPPEAPKGKKKK

• Full Name: methionyl-tRNA synthetase
• Calculated Molecular Weight: 101 kDa
• Observed Molecular Weight: 97-101 kDa
• GenBank Accession Number: BC002384
• Gene Symbol: MARS
• Gene ID (NCBI): 4141
• RRID: AB_2918508
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P56192
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.