Tested Applications
| Positive WB detected in | mouse testis tissue, human brain tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16590-1-AP targets MED31 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9008 Product name: Recombinant human MED31 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-131 aa of BC012539 Sequence: MAAAVAMETDDAGNRLRFQLELEFVQCLANPNYLNFLAQRGYFKDKAFVNYLKYLLYWKDPEYAKYLKYPQCLHMLELLQYEHFRKELVNAQCAKFIDEQQILHWQHYSRKRMRLQQALAEQQQQNNTSGK Predict reactive species |
| Full Name | mediator complex subunit 31 |
| Calculated Molecular Weight | 131 aa, 16 kDa |
| Observed Molecular Weight | 16 kDa |
| GenBank Accession Number | BC012539 |
| Gene Symbol | MED31 |
| Gene ID (NCBI) | 51003 |
| RRID | AB_2142447 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y3C7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MED31, also named as SOH1 or CGI-125, is a 131 amino acid protein, which belongs to the Mediator complex subunit 31 family. MED31 localizes in the nucleus. MED31 as a component of the Mediator complex, a coactivator is involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED31 functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MED31 antibody 16590-1-AP | Download protocol |
| WB protocol for MED31 antibody 16590-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
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| Species | Application | Title |
|---|---|---|
Dev Biol The Mediator complex protein Med31 is required for embryonic growth and cell proliferation during mammalian development. |
Reviews
What customers say
Both reviews rate the antibody 5 stars for Western blot in mouse cardiac samples. Users reported clear, good bands at 1:500 and 1:1000 dilutions, indicating reliable performance for detecting the target in mouse cardiomyocytes and heart tissue.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Anh (Verified Customer) (07-15-2021) | very clear bands
|
siting (Verified Customer) (02-17-2021) | good band
 |
