CoraLite® Plus 488-conjugated METTL2 Recombinant antibody

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  IF Staining of MCF-7 using CL488-84257

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 METTL2 antibody (CL488-84257, Clone: 241490A3 ) at dilution of 1:200.

METTL2 Uni-rAb^TM Recombinant Antibody for IF/ICC

Cat No. CL488-84257

Clone No.241490A3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

METTL2B, 241490A3, EC:2.1.1.-, methyltransferase like 2B, Methyltransferase-like protein 2B

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Tested Applications

• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84257 targets METTL2 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: METTL2 fusion protein Ag10410
• Full Name: methyltransferase like 2B
• Calculated Molecular Weight: 313 aa, 36 kDa
• Observed Molecular Weight: 36~43 kDa
• GenBank Accession Number: BC107586
• Gene Symbol: METTL2B
• Gene ID (NCBI): 55798
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6P1Q9
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

METTL2A and METTL2B are required for the threonyl and argininyl tRNA methylation. METTL2 methyltransferase is responsible for 3-methylcytosine modification of arginine tRNAs in mammals. METTL2A and METTL2B are in higher homology 99%. This antibody detects both METTL2A and METTL2B.