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METTL3 Polyclonal antibody
METTL3 Polyclonal Antibody for IHC, IP, WB, ELISA
Cat no : 15073-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HT-1080 cells, HeLa cells, A549 cells, Jurkat cells, NCCIT cells, HepG2 cells, Neuro-2a cells, COS-7 cells, rat testis tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human renal cell carcinoma tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:750-1:3000|
|Sample-dependent, check data in validation data gallery|
15073-1-AP targets METTL3 in WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with Human, Mouse, Monkey, Rat samples.
|Tested Reactivity||Human, Mouse, Monkey, Rat|
|Cited Reactivity||human, mouse, rat, chicken, drosophila, pig, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||METTL3 fusion protein Ag7110|
|Full Name||methyltransferase like 3|
|Calculated molecular weight||64 kDa|
|Observed molecular weight||65-70 kDa|
|GenBank accession number||BC001650|
|Gene ID (NCBI)||56339|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.
METTL3 regulates heterochromatin in mouse embryonic stem cells.
The RNA m6A reader YTHDC1 silences retrotransposons and guards ES cell identity.
mRNA circularization by METTL3-eIF3h enhances translation and promotes oncogenesis.
N(6)-methyladenosine-dependent regulation of messenger RNA stability.
Stem cells. m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation.
RNA-Methylation-Dependent RNA Processing Controls the Speed of the Circadian Clock.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Biao (Verified Customer) (12-23-2019)
This antibody is quite specific and gives strong signal.
Shuliang (Verified Customer) (10-14-2019)
Mattia (Verified Customer) (08-26-2019)
Clear band at expected size. Multiple unspecific bands at lower Kda. 1:1000 primary antibody dilution, blocked in 5% milk. Primary incubated overnight.