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METTL3 Polyclonal antibody
METTL3 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Monkey, Rat and More (9)
Applications
WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA
Conjugate
Unconjugated
325
Cat no : 15073-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HT-1080 cells, HeLa cells, mouse testis tissue, Jurkat cells, NCCIT cells, Neuro-2a cells, HepG2 cells, COS-7 cells, rat testis tissue, A549 cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human renal cell carcinoma tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB |
Immunohistochemistry (IHC) | IHC : 1:750-1:3000 |
Sample-dependent, check data in validation data gallery |
Product Information
15073-1-AP targets METTL3 in WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with Human, Mouse, Monkey, Rat samples.
Tested Reactivity | Human, Mouse, Monkey, Rat |
Cited Reactivity | human, mouse, rat, goat, chicken, monkey, zebrafish, pig, drosophila |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | METTL3 fusion protein Ag7110 |
Full Name | methyltransferase like 3 |
Calculated molecular weight | 64 kDa |
Observed molecular weight | 65-70 kDa |
GenBank accession number | BC001650 |
Gene symbol | METTL3 |
Gene ID (NCBI) | 56339 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is a component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for METTL3 antibody 15073-1-AP | Download protocol |
IHC protocol for METTL3 antibody 15073-1-AP | Download protocol |
IP protocol for METTL3 antibody 15073-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
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Nature The RNA m6A reader YTHDC1 silences retrotransposons and guards ES cell identity.
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Nature mRNA circularization by METTL3-eIF3h enhances translation and promotes oncogenesis.
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Science Stem cells. m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation.
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Cell RNA-Methylation-Dependent RNA Processing Controls the Speed of the Circadian Clock.
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH PK (Verified Customer) (03-20-2023) | Good
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FH Biao (Verified Customer) (12-23-2019) | This antibody is quite specific and gives strong signal.
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FH Shuliang (Verified Customer) (10-14-2019) | Good products
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FH Mattia (Verified Customer) (08-26-2019) | Clear band at expected size. Multiple unspecific bands at lower Kda. 1:1000 primary antibody dilution, blocked in 5% milk. Primary incubated overnight.
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