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- KD/KO Validated
METTL3 Polyclonal Antibody for IHC, IP, WB, ELISA
Cat no : 15073-1-AP
IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8
|Positive WB detected in||Neuro-2a cells, HEK-293 cells, HepG2 cells, HeLa cells, A549 cells, Jurkat cells, NCCIT cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15073-1-AP targets METTL3 in IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.
|Reactivity||human, mouse, rat|
|Cited Species||drosophila, human, mouse, rat, swine, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||METTL3 fusion protein Ag7110|
|Full Name||methyltransferase like 3|
|Calculated molecular weight||64 kDa|
|Observed molecular weight||65-70 kDa|
|GenBank accession number||BC001650|
|Gene ID (NCBI)||56339|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20oC. Aliquoting is unnecessary for -20oC storage.|
METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.
Mettl3 Deficiency Sustains Long-Chain Fatty Acid Absorption through Suppressing Traf6-Dependent Inflammation Response.
J Biol Chem
HIV-1 envelope proteins up-regulate N6-methyladenosine levels of cellular RNA independently of viral replication.
Int J Mol Sci
Mettl3 Regulates Osteogenic Differentiation and Alternative Splicing of Vegfa in Bone Marrow Mesenchymal Stem Cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Biao Ma (12-23-2019)
This antibody is quite specific and gives strong signal.
Shuliang Chen (10-14-2019)
Mattia Ficarelli (08-26-2019)
Clear band at expected size. Multiple unspecific bands at lower Kda. 1:1000 primary antibody dilution, blocked in 5% milk. Primary incubated overnight.