• Featured Product
  • KD/KO Validated

METTL3 Polyclonal antibody

METTL3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Monkey, Rat and More (9)

Applications

WB, IP, IF, RIP, IHC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 15073-1-AP

Synonyms

IME4, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8



Tested Applications

Positive WB detected inHEK-293 cells, HT-1080 cells, HeLa cells, mouse testis tissue, Jurkat cells, NCCIT cells, Neuro-2a cells, HepG2 cells, COS-7 cells, rat testis tissue, A549 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman renal cell carcinoma tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:750-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15073-1-AP targets METTL3 in WB, IP, IF, RIP, IHC, CoIP, ChIP, ELISA applications and shows reactivity with Human, Mouse, Monkey, Rat samples.

Tested Reactivity Human, Mouse, Monkey, Rat
Cited Reactivityhuman, mouse, rat, goat, chicken, monkey, zebrafish, pig, drosophila
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen METTL3 fusion protein Ag7110
Full Name methyltransferase like 3
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC001650
Gene Symbol METTL3
Gene ID (NCBI) 56339
RRIDAB_2142033
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is a component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.

Protocols

Product Specific Protocols
WB protocol for METTL3 antibody 15073-1-APDownload protocol
IHC protocol for METTL3 antibody 15073-1-APDownload protocol
IF protocol for METTL3 antibody 15073-1-APDownload protocol
IP protocol for METTL3 antibody 15073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

METTL3 regulates heterochromatin in mouse embryonic stem cells.

Authors - Wenqi Xu
  • KO Validated
mouseWB

Nature

The RNA m6A reader YTHDC1 silences retrotransposons and guards ES cell identity.

Authors - Jiadong Liu
  • KO Validated
humanWB

Nature

mRNA circularization by METTL3-eIF3h enhances translation and promotes oncogenesis.

Authors - Junho Choe
  • KD Validated
humanWB

Nature

N(6)-methyladenosine-dependent regulation of messenger RNA stability.

Authors - Wang Xiao X
  • KD Validated
humanWB

Mol Cancer

A positive feedback circuit driven by m6A-modified circular RNA facilitates colorectal cancer liver metastasis

Authors - Kaixuan Zeng
  • KD Validated
mouseWB,IF

Science

Stem cells. m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation.

Authors - Shay Geula
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Fanpeng (Verified Customer) (03-14-2024)

This antibody works well in my WB and IHC/IF analysis using multiple cell lines and brain tissues.

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:2000 WB, 1:200 IHC/IF
  • Cell Tissue Type: human brain/mouse brain/rat brain/N2a/M17/BV2/primary mouse brain cells
FH

PK (Verified Customer) (03-20-2023)

Good

  • Applications: Western Blot
  • Primary Antibody Dilution: 1000
  • Cell Tissue Type: AC16
METTL3 Antibody Western Blot validation (1000 dilution) in AC16 (Cat no:15073-1-AP)
FH

Biao (Verified Customer) (12-23-2019)

This antibody is quite specific and gives strong signal.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: HCT-116
FH

Shuliang (Verified Customer) (10-14-2019)

Good products

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: HEK293T cells
METTL3 Antibody Western Blot, validation (1:3000 dilution) in HEK293T cells (Cat no:15073-1-AP)
FH

Mattia (Verified Customer) (08-26-2019)

Clear band at expected size. Multiple unspecific bands at lower Kda. 1:1000 primary antibody dilution, blocked in 5% milk. Primary incubated overnight.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa, HEK293T