• Featured Product
  • KD/KO Validated

METTL3 Polyclonal antibody

METTL3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, RIP, IP, IHC, IF, CoIP, ChIP

Conjugate

Unconjugated

Cat no : 15073-1-AP

Synonyms

IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, A549 cells, HeLa cells, HT-1080 cells, Jurkat cells, NCCIT cells, Neuro-2a cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy METTL3 Ready-To-Use IHC Kit. METTL3 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15073-1-AP targets METTL3 in WB, RIP, IP, IHC, IF, CoIP, ChIP applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity drosophila, human, mouse, pig, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen METTL3 fusion protein Ag7110
Full Name methyltransferase like 3
Calculated molecular weight 64 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC001650
Gene symbol METTL3
Gene ID (NCBI) 56339
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.

Protocols

Product Specific Protocols
WB protocol for METTL3 antibody 15073-1-APDownload protocol
IHC protocol for METTL3 antibody 15073-1-APDownload protocol
IP protocol for METTL3 antibody 15073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Immunol

Mettl3 Deficiency Sustains Long-Chain Fatty Acid Absorption through Suppressing Traf6-Dependent Inflammation Response.

Authors - Xin Zong
humanWB

J Biol Chem

HIV-1 envelope proteins up-regulate N6-methyladenosine levels of cellular RNA independently of viral replication.

Authors - Nagaraja Tirumuru
ratWB

Int J Mol Sci

Mettl3 Regulates Osteogenic Differentiation and Alternative Splicing of Vegfa in Bone Marrow Mesenchymal Stem Cells.

Authors - Cheng Tian
  • KD Validated
mouseWB,IHC,IF

Cell Death Dis

m6A methylation controls pluripotency of porcine induced pluripotent stem cells by targeting SOCS3/JAK2/STAT3 pathway in a YTHDF1/YTHDF2-orchestrated manner.

Authors - Ruifan Wu
  • KD Validated
humanWB,IHC

Oncogene

Dynamic m6A mRNA methylation reveals the role of METTL3-m6A-CDCP1 signaling axis in chemical carcinogenesis.

Authors - Fan Yang
  • KO Validated
WB

Autophagy

METTL3 and ALKBH5 oppositely regulate m6A modification of TFEB mRNA, which dictates the fate of hypoxia/reoxygenation-treated cardiomyocytes.

Authors - Huiwen Song
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Biao (Verified Customer) (12-23-2019)

This antibody is quite specific and gives strong signal.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: HCT-116
FH

Shuliang (Verified Customer) (10-14-2019)

Good products

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: HEK293T cells
METTL3 Antibody Western Blot, validation (1:3000 dilution) in HEK293T cells (Cat no:15073-1-AP)
FH

Mattia (Verified Customer) (08-26-2019)

Clear band at expected size. Multiple unspecific bands at lower Kda. 1:1000 primary antibody dilution, blocked in 5% milk. Primary incubated overnight.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa, HEK293T