• Featured Product
  • KD/KO Validated

METTL3 Antibody

METTL3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Publications

63

Cat no : 15073-1-AP

Synonyms

IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8



Tested Applications

Positive WB detected inNeuro-2a cells, HEK-293 cells, HepG2 cells, HeLa cells, A549 cells, Jurkat cells, NCCIT cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15073-1-AP targets METTL3 in IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species drosophila, human, mouse, rat, swine, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen METTL3 fusion protein Ag7110
Full Name methyltransferase like 3
Calculated molecular weight 64 kDa
Observed molecular weight 65-70 kDa
GenBank accession number BC001650
Gene symbol METTL3
Gene ID (NCBI) 56339
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.

Protocols

Product Specific Protocols
WB protocol for METTL3 antibody 15073-1-APDownload protocol
IHC protocol for METTL3 antibody 15073-1-APDownload protocol
IP protocol for METTL3 antibody 15073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Immunol

Mettl3 Deficiency Sustains Long-Chain Fatty Acid Absorption through Suppressing Traf6-Dependent Inflammation Response.

Authors - Xin Zong
humanWB

J Biol Chem

HIV-1 envelope proteins up-regulate N6-methyladenosine levels of cellular RNA independently of viral replication.

Authors - Nagaraja Tirumuru
ratWB

Int J Mol Sci

Mettl3 Regulates Osteogenic Differentiation and Alternative Splicing of Vegfa in Bone Marrow Mesenchymal Stem Cells.

Authors - Cheng Tian
  • KD Validated
mouseWB,IHC,IF

Cell Death Dis

m6A methylation controls pluripotency of porcine induced pluripotent stem cells by targeting SOCS3/JAK2/STAT3 pathway in a YTHDF1/YTHDF2-orchestrated manner.

Authors - Ruifan Wu
  • KD Validated
mouseWB,IHC,IF

Cell Death Dis

m6A methylation controls pluripotency of porcine induced pluripotent stem cells by targeting SOCS3/JAK2/STAT3 pathway in a YTHDF1/YTHDF2-orchestrated manner.

Authors - Ruifan Wu
  • KD Validated
mouseWB,IHC,IF

Cell Death Dis

m6A methylation controls pluripotency of porcine induced pluripotent stem cells by targeting SOCS3/JAK2/STAT3 pathway in a YTHDF1/YTHDF2-orchestrated manner.

Authors - Ruifan Wu
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Biao Ma (12-23-2019)

This antibody is quite specific and gives strong signal.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: HCT-116
No image submitted with review
FH

Shuliang Chen (10-14-2019)

Good products

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: HEK293T cells
METTL3 Antibody Western Blot, validation (1:3000 dilution) in HEK293T cells (Cat no:15073-1-AP)
FH

Mattia Ficarelli (08-26-2019)

Clear band at expected size. Multiple unspecific bands at lower Kda. 1:1000 primary antibody dilution, blocked in 5% milk. Primary incubated overnight.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa, HEK293T
No image submitted with review