Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HepG2 cells, A549 cells, HeLa cells, HT-1080 cells, Jurkat cells, NCCIT cells, Neuro-2a cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15073-1-AP targets METTL3 in WB, RIP, IP, IHC, IF, CoIP, ChIP applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||drosophila, human, mouse, pig, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||METTL3 fusion protein Ag7110|
|Full Name||methyltransferase like 3|
|Calculated molecular weight||64 kDa|
|Observed molecular weight||65-70 kDa|
|GenBank accession number||BC001650|
|Gene ID (NCBI)||56339|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.
Mettl3 Deficiency Sustains Long-Chain Fatty Acid Absorption through Suppressing Traf6-Dependent Inflammation Response.
J Biol Chem
HIV-1 envelope proteins up-regulate N6-methyladenosine levels of cellular RNA independently of viral replication.
Int J Mol Sci
Mettl3 Regulates Osteogenic Differentiation and Alternative Splicing of Vegfa in Bone Marrow Mesenchymal Stem Cells.
Cell Death Dis
m6A methylation controls pluripotency of porcine induced pluripotent stem cells by targeting SOCS3/JAK2/STAT3 pathway in a YTHDF1/YTHDF2-orchestrated manner.
Dynamic m6A mRNA methylation reveals the role of METTL3-m6A-CDCP1 signaling axis in chemical carcinogenesis.
METTL3 and ALKBH5 oppositely regulate m6A modification of TFEB mRNA, which dictates the fate of hypoxia/reoxygenation-treated cardiomyocytes.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Biao (Verified Customer) (12-23-2019)
This antibody is quite specific and gives strong signal.
Shuliang (Verified Customer) (10-14-2019)
Mattia (Verified Customer) (08-26-2019)
Clear band at expected size. Multiple unspecific bands at lower Kda. 1:1000 primary antibody dilution, blocked in 5% milk. Primary incubated overnight.