Tested Applications
Positive IF-P detected in | mouse testis tissue |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL594-15073 targets METTL3 in applications and shows reactivity with human, mouse, rat, monkey samples.
Tested Reactivity | human, mouse, rat, monkey |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | METTL3 fusion protein Ag7110 Predict reactive species |
Full Name | methyltransferase like 3 |
Calculated Molecular Weight | 64 kDa |
Observed Molecular Weight | 65-70 kDa |
GenBank Accession Number | BC001650 |
Gene Symbol | METTL3 |
Gene ID (NCBI) | 56339 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Antigen affinity Purification |
UNIPROT ID | Q86U44 |
Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is a component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.
Protocols
Product Specific Protocols | |
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IF protocol for CL594 METTL3 antibody CL594-15073 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |