CoraLite®594-conjugated METTL3 Polyclonal antibody

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  IF Staining of mouse testis using CL594-15073

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-15073) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

METTL3 Polyclonal Antibody for IF-P

Cat No. CL594-15073

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

IF-P

Synonyms

EC:2.1.1.348, hMETTL3, Methyltransferase-like protein 3, MT A70, MTA70

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Tested Applications

• Positive IF-P detected in: mouse testis tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-15073 targets METTL3 in IF-P applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: METTL3 fusion protein Ag7110
• Full Name: methyltransferase like 3
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC001650
• Gene Symbol: METTL3
• Gene ID (NCBI): 56339
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q86U44
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is a component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.