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CoraLite®488-conjugated METTL3 Monoclonal antibody

METTL3 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

2D8H1

Cat no : CL488-67733

Synonyms

IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8



Tested Applications

Positive IF detected inmouse testis tissue
For other applications, we recommend the unconjugated version of this antibody, 67733-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

CL488-67733 targets METTL3 in IF applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen METTL3 fusion protein Ag7110
Full Name methyltransferase like 3
Calculated molecular weight 64 kDa
Observed molecular weight70 kDa
GenBank accession numberBC001650
Gene symbol METTL3
Gene ID (NCBI) 56339
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.

Protocols

Product Specific Protocols
IF protocol for CL488 METTL3 antibody CL488-67733Download protocol
Standard Protocols
Click here to view our Standard Protocols