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METTL3 Monoclonal antibody
METTL3 Monoclonal Antibody for IF, IHC, WB, ELISA
Cat no : 67733-1-Ig
Validation Data Gallery
|Positive WB detected in||HeLa cells, A549 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells|
|Positive IHC detected in||human lung cancer tissue, human urothelial carcinoma tissue, human colon cancer tissue, human oesophagus cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse testis tissue|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
67733-1-Ig targets METTL3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, mouse samples.
|Tested Reactivity||Human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||METTL3 fusion protein Ag7110|
|Full Name||methyltransferase like 3|
|Calculated molecular weight||64 kDa|
|Observed molecular weight||65-70 kDa|
|GenBank accession number||BC001650|
|Gene ID (NCBI)||56339|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.
Therapeutic targeting m6A-guided miR-146a-5p signaling contributes to the melittin-induced selective suppression of bladder cancer.
Am J Respir Cell Mol Biol
The Combined Effects of Circular RNA Methylation Promote Pulmonary Fibrosis.
Evid Based Complement Alternat Med
m6A RNA Methylation Regulators Contribute to Predict and as a Therapy Target of Pulmonary Fibrosis.
Int J Mol Sci
Reduction of Methyltransferase-like 3-Mediated RNA N6-Methyladenosine Exacerbates the Development of Psoriasis Vulgaris in Imiquimod-Induced Psoriasis-like Mouse Model
NRP1 contributes to stemness and potentiates radioresistance via WTAP-mediated m6A methylation of Bcl-2 mRNA in breast cancer
Matr3 reshapes m6A modification complex to alleviate macrophage inflammation during atherosclerosis