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  • KD/KO Validated

METTL3 Monoclonal antibody

METTL3 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (2)

Applications

WB, IP, IF, FC, RIP, IHC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

2D8H1

Cat no : 67733-1-Ig

Synonyms

IME4, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8



Tested Applications

Positive WB detected inHeLa cells, A549 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman lung cancer tissue, human urothelial carcinoma tissue, human colon cancer tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse testis tissue
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67733-1-Ig targets METTL3 in WB, IP, IF, FC, RIP, IHC, CoIP, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen METTL3 fusion protein Ag7110
Full Name methyltransferase like 3
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC001650
Gene Symbol METTL3
Gene ID (NCBI) 56339
RRIDAB_2918502
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.

Protocols

Product Specific Protocols
WB protocol for METTL3 antibody 67733-1-IgDownload protocol
IHC protocol for METTL3 antibody 67733-1-IgDownload protocol
IF protocol for METTL3 antibody 67733-1-IgDownload protocol
FC protocol for METTL3 antibody 67733-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

EMBO Rep

DDX5 inhibits inflammation by modulating m6A levels of TLR2/4 transcripts during bacterial infection

Authors - Jian Xu
humanIP

Cancer Lett

Therapeutic targeting m6A-guided miR-146a-5p signaling contributes to the melittin-induced selective suppression of bladder cancer.

Authors - Rucheng Yan
humanWB,IF,IP

J Invest Dermatol

NPTX2 Promotes EMT in Cutaneous Squamous Cell Carcinoma via Mettl3-Mediated m6A Methylation of Snail

Authors - Tong Kang
human,mouseWB,IF

Am J Respir Cell Mol Biol

The Combined Effects of Circular RNA Methylation Promote Pulmonary Fibrosis.

Authors - Sha Wang
  • KD Validated
human,mouseIHC

Evid Based Complement Alternat Med

m6A RNA Methylation Regulators Contribute to Predict and as a Therapy Target of Pulmonary Fibrosis.

Authors - Meng-Sheng Deng
mouseWB,IHC

Int J Mol Sci

Reduction of Methyltransferase-like 3-Mediated RNA N6-Methyladenosine Exacerbates the Development of Psoriasis Vulgaris in Imiquimod-Induced Psoriasis-like Mouse Model

Authors - Yanan Wang
  • KO Validated