Multi-rAb® METTL5 Multi-Recombinant antibody

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  WB analysis using RMX00070

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00070 (METTL5 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using RMX00070

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using RMX00070 (METTL5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using RMX00070

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using METTL5 antibody (RMX00070) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

METTL5 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. RMX00070

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:2.1.1.-, HSPC133, methyltransferase like 5, Methyltransferase-like protein 5, rRNA N6-adenosine-methyltransferase METTL5

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Tested Applications

• Positive WB detected in: Ramos cells, THP-1 cells
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00070 targets METTL5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: methyltransferase like 5
• Calculated Molecular Weight: 209 aa, 24 kDa
• Observed Molecular Weight: 24-27 kDa
• GenBank Accession Number: BC000921
• Gene Symbol: METTL5
• Gene ID (NCBI): 29081
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: Q9NRN9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

METTL5 is a member of the METTL family of S-adenosyl-methionine-dependent methyltransferases that adds m6A to a specific site, nucleotide A-1832 of 18S rRNA, hereafter referred to as m6A1832. METTL5 is localized in the nucleolus and cytoplasm.