MICAL2 Polyclonal antibody, PBS Only

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  WB analysis using 13965-1-AP (same clone as 13965-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13965-1-AP (MICAL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13965-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 13965-1-AP (same clone as 13965-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13965-1-AP (MICAL2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13965-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 13965-1-AP (same clone as 13965-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13965-1-AP (MICAL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13965-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 13965-1-AP (same clone as 13965-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13965-1-AP (MICAL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13965-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 13965-1-AP (same clone as 13965-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13965-1-AP (MICAL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13965-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 13965-1-AP (same clone as 13965-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using MICAL2 antibody (13965-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13965-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 13965-1-AP (same clone as 13965-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MICAL2 antibody (13965-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 13965-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

MICAL2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 13965-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

13965-1-PBS targets MICAL2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4950

  Product name: Recombinant human MICAL2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 771-1124 aa of BC044577

  Sequence: KAAHLASMFGHGDFPQNKLLSKGLSHTHPPSPPSRLPSPDPAASSSPSTVDSASPARKEKKSPSGFHFHPSHLRTVHPQLTVGKVSSGIGAAAEVLVNLYMNDHRPKAQATSPDLESMRKSFPLNLGGSDTCYFCKKRVYVMERLSAEGHFFHRECFRCSICATTLRLAAYTFDCDEGKFYCKPHFIHCKTNSKQRKRRAELKQQREEEATWQEQEAPRRDTPTESSCAVAAIGTLEGSPPVHFSLPVLHPLLG

• Full Name: microtubule associated monoxygenase, calponin and LIM domain containing 2
• Calculated Molecular Weight: 127 kDa
• Observed Molecular Weight: 95 kDa, 112 kDa
• GenBank Accession Number: BC044577
• Gene Symbol: MICAL2
• Gene ID (NCBI): 9645
• RRID: AB_2143764
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O94851
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions. There are three known isoforms, MICAL1, MICAL2, and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL2 has three conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain, and a Lin11, Isl-1, and Mec-3 (LIM) domain. It has been demonstrated that MICAL2 could regulate actin stress fibers and is required for normal actin organization. In addition, MICAL2-PV, a novel splicing variant of MICAL2, has been reported to be involved in cancer progression of prostate cancer. This antibody can recognize both MICAL2 and MICAL2-PV. This antibody recognizes various isoforms of MICAL2 around 90-95 kDa or 109-112 kDa.