MMADHC Polyclonal antibody

MMADHC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23191-1-AP

Synonyms

C2orf25, cblD, CL25022, MMADHC, My011



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, NIH3T3 cells
Positive IHC detected inhuman skeletal muscle tissue, human heart tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23191-1-AP is MMADHC Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMADHC fusion protein Ag19343
Full Name methylmalonic aciduria (cobalamin deficiency) cblD type, with homocystinuria
Calculated Molecular Weight 296 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC022859
Gene Symbol MMADHC
Gene ID (NCBI) 27249
RRIDAB_2879229
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MMADHC is a mitochondrial protein that is involved in an early step of vitamin B12 metabolism. Vitamin B12 (cobalamin) is essential for normal development and survival in humans. MMADHC is a 296-amino acids (32.9 kDa) protein with an N-terminal disordered region (amino acids 1-107) containing a potential mitochondrial leader sequence (MLS; amino acids 1-12), and a C-terminal Nitro Reductase-like domain (NTR; amino acids 108-296). Mutations in this gene cause methylmalonic aciduria and homocystinuria type cblD (MMADHC), a disorder of cobalamin metabolism that is characterized by decreased levels of the coenzymes adenosylcobalamin and methylcobalamin.

Protocols

Product Specific Protocols
WB protocol for MMADHC antibody 23191-1-APDownload protocol
IHC protocol for MMADHC antibody 23191-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols