Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, MCF-7 cells, NIH3T3 cells |
| Positive IHC detected in | human skeletal muscle tissue, human heart tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23191-1-AP targets MMADHC in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19343 Product name: Recombinant human MMADHC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 41-291 aa of BC022859 Sequence: ESHVAAAPPDICSRTVWPDETMGPFGPQDQRFQLPGNIGFDCHLNGTASQKKSLVHKTLPDVLAEPLSSERHEFVMAQYVNEFQGNDAPVEQEINSAETYFESARVECAIQTCPELLRKDFESLFPEVANGKLMILTVTQKTKNDMTVWSEEVEIEREVLLEKFINGAKEICYALRAEGYWADFIDPSSGLAFFGPYTNNTLFETDERYRHLGFSVDDLGCCKVIRHSLWGTHVVVGSIFTNATPDSHIMK Predict reactive species |
| Full Name | methylmalonic aciduria (cobalamin deficiency) cblD type, with homocystinuria |
| Calculated Molecular Weight | 296 aa, 33 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC022859 |
| Gene Symbol | MMADHC |
| Gene ID (NCBI) | 27249 |
| RRID | AB_2879229 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H3L0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MMADHC is a mitochondrial protein that is involved in an early step of vitamin B12 metabolism. Vitamin B12 (cobalamin) is essential for normal development and survival in humans. MMADHC is a 296-amino acids (32.9 kDa) protein with an N-terminal disordered region (amino acids 1-107) containing a potential mitochondrial leader sequence (MLS; amino acids 1-12), and a C-terminal Nitro Reductase-like domain (NTR; amino acids 108-296). Mutations in this gene cause methylmalonic aciduria and homocystinuria type cblD (MMADHC), a disorder of cobalamin metabolism that is characterized by decreased levels of the coenzymes adenosylcobalamin and methylcobalamin.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MMADHC antibody 23191-1-AP | Download protocol |
| WB protocol for MMADHC antibody 23191-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MMADHC Polyclonal antibody (Cat# 23191-1-AP) has 2 citations. One appears in Cell Reports (2026), studying how Afg3l2 couples mitochondrial vitamin B12 trafficking to amino acid metabolism in hematopoietic stem cell homeostasis. The other is in Differentiation (2025), exploring genetic tools for cobalamin metabolism disorders impacting mammalian development. Both studies used the antibody for WB and CoIP applications in mouse models.
| Species | Application | Title |
|---|---|---|
Cell Rep Afg3l2 couples mitochondrial vitamin B12 trafficking to amino acid metabolism to safeguard hematopoietic stem cell homeostasis. | ||
Differentiation New genetic tools to define the pathophysiology of inborn errors of cobalamin metabolism impacting mammalian development |
