Filter: AllWBIHC

WB analysis using 23191-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 23191-1-AP (MMADHC antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 23191-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 23191-1-AP (MMADHC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 23191-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 23191-1-AP (MMADHC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human skeletal muscle using 23191-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human skeletal muscle using 23191-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human heart using 23191-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 23191-1-AP (MMADHC antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 23191-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 23191-1-AP (MMADHC antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 23191-1-AP
Immunohistochemical analysis of paraffin-embedded human heart slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 23191-1-AP
Immunohistochemical analysis of paraffin-embedded human heart slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human pancreas using 23191-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 23191-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:50 (under 40x lens).

MMADHC Polyclonal antibody

Name: MMADHC Polyclonal antibody
Brand: Proteintech
SKU: 23191-1-AP

MMADHC Polyclonal Antibody for WB, IHC, ELISA

Cat No. 23191-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

C2orf25, cblD, CL25022, MMADHC, My011

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, MCF-7 cells, NIH3T3 cells
Positive IHC detected in	human skeletal muscle tissue, human heart tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23191-1-AP targets MMADHC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag19343 Product name: Recombinant human MMADHC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 41-291 aa of BC022859 Sequence: ESHVAAAPPDICSRTVWPDETMGPFGPQDQRFQLPGNIGFDCHLNGTASQKKSLVHKTLPDVLAEPLSSERHEFVMAQYVNEFQGNDAPVEQEINSAETYFESARVECAIQTCPELLRKDFESLFPEVANGKLMILTVTQKTKNDMTVWSEEVEIEREVLLEKFINGAKEICYALRAEGYWADFIDPSSGLAFFGPYTNNTLFETDERYRHLGFSVDDLGCCKVIRHSLWGTHVVVGSIFTNATPDSHIMK Predict reactive species
Full Name	methylmalonic aciduria (cobalamin deficiency) cblD type, with homocystinuria
Calculated Molecular Weight	296 aa, 33 kDa
Observed Molecular Weight	33 kDa
GenBank Accession Number	BC022859
Gene Symbol	MMADHC
Gene ID (NCBI)	27249
RRID	AB_2879229
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H3L0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MMADHC is a mitochondrial protein that is involved in an early step of vitamin B12 metabolism. Vitamin B12 (cobalamin) is essential for normal development and survival in humans. MMADHC is a 296-amino acids (32.9 kDa) protein with an N-terminal disordered region (amino acids 1-107) containing a potential mitochondrial leader sequence (MLS; amino acids 1-12), and a C-terminal Nitro Reductase-like domain (NTR; amino acids 108-296). Mutations in this gene cause methylmalonic aciduria and homocystinuria type cblD (MMADHC), a disorder of cobalamin metabolism that is characterized by decreased levels of the coenzymes adenosylcobalamin and methylcobalamin.