MMP1 Polyclonal antibody

MMP1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 26585-1-AP

Synonyms

CLG, CLGN, Fibroblast collagenase, Interstitial collagenase, Matrix metalloproteinase 1, MMP 1, MMP1

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Validation Data Gallery

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  WB analysis of A375 using 26585-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 26585-1-AP (MMP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 26585-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26585-1-AP (MMP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 26585-1-AP

  IP result of anti-MMP1 (IP:26585-1-AP, 4ug; Detection:26585-1-AP 1:300) with Raji cells lysate 4000ug.

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  IHC staining of human cervical cancer using 26585-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 26585-1-AP (MMP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 26585-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 26585-1-AP (MMP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 26585-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26585-1-AP (MMP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 26585-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26585-1-AP (MMP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A375 cells, NIH/3T3 cells
• Positive IP detected in: Raji cells
• Positive IHC detected in: human cervical cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

The immunogen of 26585-1-AP is MMP1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MMP1 fusion protein Ag25073
• Full Name: matrix metallopeptidase 1 (interstitial collagenase)
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC013875
• Gene Symbol: MMP1
• Gene ID (NCBI): 4312
• RRID: AB_2880564
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MMP1, also named as CLG, belongs to the peptidase M10A family. MMP is the main enzyme that cleaves fibrillar collagen, namely types I, II, III, VII, and X.It is involved in cell migration and invasion, and is frequently up-regulated in cancer cells. It can be cleavage to two major forms (22 kDa and 27 kDa) by undergoing autolytic,and the minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.The sizes of pro-MMP-1 and active MMP-1 from human synovial fibroblasts have been reported as 52 to 56 kDa, depending on glycosylation, and 41 to 45 kDa, respectively.In addition ,a band of 62-kDa observed in the current study is pro-MMp-1(PMID:9418730).

Publications

Species	Application	Title
human,mouse	WB,IHC,IP	Breast Cancer Res Treat; Exosomal MMP-1 transfers metastasis potential in triple-negative breast cancer through PAR1-mediated EMT.; Authors - Yihui Zhu
human	WB	Front Genet; Exhausting circ_0136474 and Restoring miR-766-3p Attenuate Chondrocyte Oxidative Injury in IL-1β-Induced Osteoarthritis Progression Through Regulating DNMT3A.; Authors - Haiquan Zhu
		Mol Med Rep; Icariin inhibits MMP‑1, MMP‑3 and MMP‑13 expression through MAPK pathways in IL‑1β‑stimulated SW1353 chondrosarcoma cells.; Authors - Li Zeng