MPO Monoclonal antibody

• Featured Product
• KD/KO Validated

MPO Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgA

Reactivity

human, rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

12

CloneNo.

4C11F6

Cat no : 66177-1-Ig

Synonyms

MPO, myeloperoxidase

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Validation Data Gallery

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  WB analysis of HL-60 using 66177-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66177-1-Ig (MPO antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66177-1-Ig

  WB result of MPO antibody (66177-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MPO transfected HeLa cells.

• 

  IHC staining of human liver using 66177-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with MPO antibody included
• 

  IHC staining of human liver using 66177-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with MPO antibody included
• 

  IHC staining of human tonsillitis using 66177-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with MPO antibody included
• 

  IHC staining of human tonsillitis using 66177-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with MPO antibody included
• 

  IF Staining of human tonsillitis using 66177-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66177-1-Ig (MPO antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HL-60 cells
• Positive IHC detected in: human liver tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human tonsillitis tissue

Planning an IHC experiment? We recommend our IHCeasy MPO Ready-To-Use IHC Kit. MPO primary antibody included.

Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below
• IHC: See 6 publications below
• IF: See 5 publications below

Product Information

66177-1-Ig targets MPO in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, bovine, cow
• Host / Isotype: Mouse / IgA
• Class: Monoclonal
• Type: Antibody
• Immunogen: MPO fusion protein Ag17564
• Full Name: myeloperoxidase
• Calculated molecular weight: 745 aa, 84 kDa
• Observed molecular weight: 90 kDa
• GenBank accession number: BC130476
• Gene symbol: MPO
• Gene ID (NCBI): 4353
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	IF,WB	Cell Death Dis; Neutrophil extracellular traps impair intestinal barrier functions in sepsis by regulating TLR9-mediated endoplasmic reticulum stress pathway.; Authors - Shilong Sun
cow	IF	Front Vet Sci; Effect of BLV Infection on the Immune Function of Polymorphonuclear Neutrophil in Dairy Cows; Authors - Guanxin Lv
bovine	IF	Biol Trace Elem Res; Effect of Copper, Zinc, and Selenium on the Formation of Bovine Neutrophil Extracellular Traps.; Authors - Xiechen Zhou
mouse	IHC	Am J Transl Res; VE-cadherin involved in the pulmonary microvascular endothelial cell barrier injury induced by angiotensin II through modulating the cellular apoptosis and skeletal rearrangement.; Authors - Zhiyong Wu
human	IHC	Mediators Inflamm; Dephosphorylation of Y685-VE-Cadherin Involved in Pulmonary Microvascular Endothelial Barrier Injury Induced by Angiotensin II.; Authors - Zhiyong Wu
human	IF	Front Pharmacol; The Role of Formyl Peptide Receptor 1 in Uterine Contraction During Parturition.; Authors - Chaolu Chen