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MPO Monoclonal antibody

MPO Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgA

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4C11F6

Cat no : 66177-1-Ig

Synonyms

MPO, myeloperoxidase



Tested Applications

Positive WB detected inHL-60 cells
Positive IHC detected inhuman liver tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy MPO Ready-To-Use IHC Kit. MPO primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66177-1-Ig targets MPO in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivity bovine, human
Host / Isotype Mouse / IgA
Class Monoclonal
Type Antibody
Immunogen MPO fusion protein Ag17564
Full Name myeloperoxidase
Calculated molecular weight 745 aa, 84 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC130476
Gene symbol MPO
Gene ID (NCBI) 4353
Conjugate Unconjugated
Form Liquid
Purification Method Thiophilic affinity chromatograph
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for MPO antibody 66177-1-IgDownload protocol
IHC protocol for MPO antibody 66177-1-IgDownload protocol
IF protocol for MPO antibody 66177-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF,WB

Cell Death Dis

Neutrophil extracellular traps impair intestinal barrier functions in sepsis by regulating TLR9-mediated endoplasmic reticulum stress pathway.

Authors - Shilong Sun
bovineIF

Biol Trace Elem Res

Effect of Copper, Zinc, and Selenium on the Formation of Bovine Neutrophil Extracellular Traps.

Authors - Xiechen Zhou
mouseIHC

Am J Transl Res

VE-cadherin involved in the pulmonary microvascular endothelial cell barrier injury induced by angiotensin II through modulating the cellular apoptosis and skeletal rearrangement.

Authors - Zhiyong Wu
humanIHC

Mediators Inflamm

Dephosphorylation of Y685-VE-Cadherin Involved in Pulmonary Microvascular Endothelial Barrier Injury Induced by Angiotensin II.

Authors - Zhiyong Wu
humanIF

Front Pharmacol

The Role of Formyl Peptide Receptor 1 in Uterine Contraction During Parturition.

Authors - Chaolu Chen
humanIF

Front Oncol

Stromal Neutrophil Extracellular Trap Density Is an Independent Prognostic Factor for Cervical Cancer Recurrence.

Authors - Bin Yan