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MPO Monoclonal antibody

MPO Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgA

Reactivity

human, rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4C11F6

Cat no : 66177-1-Ig

Synonyms

MPO, myeloperoxidase



Tested Applications

Positive WB detected inHL-60 cells
Positive IHC detected inhuman liver tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66177-1-Ig targets MPO in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, bovine, cow
Host / Isotype Mouse / IgA
Class Monoclonal
Type Antibody
Immunogen MPO fusion protein Ag17564
Full Name myeloperoxidase
Calculated molecular weight 745 aa, 84 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC130476
Gene symbol MPO
Gene ID (NCBI) 4353
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for MPO antibody 66177-1-IgDownload protocol
IHC protocol for MPO antibody 66177-1-IgDownload protocol
IF protocol for MPO antibody 66177-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

PLoS Pathog

Prion protein signaling induces M2 macrophage polarization and protects from lethal influenza infection in mice.

Authors - Junji Chida
humanIHC

Sci Rep

Persisting and Increasing Neutrophil Infiltration Associates with Gastric Carcinogenesis and E-cadherin Downregulation.

Authors - Hualin Fu
mouseIHC

Sci Rep

Interleukin 22 attenuated angiotensin II induced acute lung injury through inhibiting the apoptosis of pulmonary microvascular endothelial cells.

Authors - Zhiyong Wu
humanIHC

Front Immunol

Leukocytes in Cerebral Thrombus Respond to Large-Vessel Occlusion in a Time-Dependent Manner and the Association of NETs With Collateral Flow.

Authors - Xi Chen
humanIHC

Front Immunol

An integrated bioinformatic investigation of mitochondrial energy metabolism genes in colon adenocarcinoma followed by preliminary validation of CPT2 in tumor immune infiltration

Authors - Zichao Cao
humanIF

Front Oncol

Stromal Neutrophil Extracellular Trap Density Is an Independent Prognostic Factor for Cervical Cancer Recurrence.

Authors - Bin Yan