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MPO Monoclonal antibody
MPO Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgA
Reactivity
human, rat and More (2)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
4C11F6
Cat no : 66177-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HL-60 cells |
Positive IHC detected in | human liver tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human tonsillitis tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 1 publications below |
IHC | See 6 publications below |
IF | See 5 publications below |
Product Information
66177-1-Ig targets MPO in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.
Tested Reactivity | human, rat |
Cited Reactivity | human, bovine, cow |
Host / Isotype | Mouse / IgA |
Class | Monoclonal |
Type | Antibody |
Immunogen | MPO fusion protein Ag17564 |
Full Name | myeloperoxidase |
Calculated molecular weight | 745 aa, 84 kDa |
Observed molecular weight | 90 kDa |
GenBank accession number | BC130476 |
Gene symbol | MPO |
Gene ID (NCBI) | 4353 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.
Protocols
Product Specific Protocols | |
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WB protocol for MPO antibody 66177-1-Ig | Download protocol |
IHC protocol for MPO antibody 66177-1-Ig | Download protocol |
IF protocol for MPO antibody 66177-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Death Dis Neutrophil extracellular traps impair intestinal barrier functions in sepsis by regulating TLR9-mediated endoplasmic reticulum stress pathway. | ||
Front Vet Sci Effect of BLV Infection on the Immune Function of Polymorphonuclear Neutrophil in Dairy Cows | ||
Biol Trace Elem Res Effect of Copper, Zinc, and Selenium on the Formation of Bovine Neutrophil Extracellular Traps. | ||
Am J Transl Res VE-cadherin involved in the pulmonary microvascular endothelial cell barrier injury induced by angiotensin II through modulating the cellular apoptosis and skeletal rearrangement. | ||
Mediators Inflamm Dephosphorylation of Y685-VE-Cadherin Involved in Pulmonary Microvascular Endothelial Barrier Injury Induced by Angiotensin II. | ||
Front Pharmacol The Role of Formyl Peptide Receptor 1 in Uterine Contraction During Parturition. |